Mechanisms of inherited deficiencies of multiple UDP-glucuronosyltransferase isoforms in two patients with Crigler-Najjar syndrome, type I

Piter J. Bosma, Jayanta Roy-Chowdhury, Tian Jun Huang, Pulak Lahiri, Ronald P J Oude Elferink, Helmuth H G Van Es, Mitchell Lederstein, Peter F. Whitington, Peter L M Jansen, Namita Roy Chowdhury

Research output: Contribution to journalArticle

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Abstract

Crigler-Najjar syndrome, type I (CN-I) is a potentially lethal disorder characterized by severe unconjugated hyperbilirubinemia resulting from a recessively inherited deficiency of hepatic UDP-glucuronosyl-transferase (UGT) activity toward bilirubin (B-UGT). Two forms of B-UGT exist in human liver. mRNAs for these two forms and that for another isoform with activity toward simple phenols (P-UGT) have unique 5′ regions, but their 3′ regions are identical. The three mRNA species are derived from a single locus; the unique 5′ regions are encoded by single unique exons and the identical 3′ regions consist of four consecutive exons that are shared by all three isoforms. In this paper, we determined genetic lesions in two CN-I patients with deficiency of hepatic B-UGT and P-UGT activities. In one patient, there was a C→T substitution in exon 4 (common region) predicting the substitution of a serine residue with a phenylalanine residue; this mutation was present in the identical region of B-UGT and P-UGT mRNAs. In the other patient, a C→T substitution in exon 2 (common region) of the B-UGT/P-UGT locus resulted in a premature stop codon. This exon (132 nt) was absent in heptic B-UGT and P-UGT mRNAs of this patient due to exon skipping during pre-mRNA processing. Sequence abnormality of three distinct mRNA species explains the abnormality of multiple UGT isoforms in these patients. Presence of identical abnormalities in the common regions of the three mRNAs is consistent with the finding that the common 3′ regions of the two B-UGT mRNAs and the P-UGT mRNA are encoded by four shared exons.

Original languageEnglish (US)
Pages (from-to)2859-2863
Number of pages5
JournalFASEB Journal
Volume6
Issue number10
StatePublished - Jul 1992

Fingerprint

Crigler-Najjar Syndrome
Glucuronosyltransferase
Uridine Diphosphate
Transferases
transferases
Protein Isoforms
exons
Exons
Messenger RNA
Substitution reactions
liver
Liver
hyperbilirubinemia
Multiple Abnormalities
loci

Keywords

  • 4-nitrophenol
  • Bilirubin
  • Crigler-Najjar syndrome
  • Exon skipping
  • Multiple isoform deficiency
  • Sequence abnormality
  • Type I
  • UDP-glucuronosyltransferase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Bosma, P. J., Roy-Chowdhury, J., Huang, T. J., Lahiri, P., Oude Elferink, R. P. J., Van Es, H. H. G., ... Chowdhury, N. R. (1992). Mechanisms of inherited deficiencies of multiple UDP-glucuronosyltransferase isoforms in two patients with Crigler-Najjar syndrome, type I. FASEB Journal, 6(10), 2859-2863.

Mechanisms of inherited deficiencies of multiple UDP-glucuronosyltransferase isoforms in two patients with Crigler-Najjar syndrome, type I. / Bosma, Piter J.; Roy-Chowdhury, Jayanta; Huang, Tian Jun; Lahiri, Pulak; Oude Elferink, Ronald P J; Van Es, Helmuth H G; Lederstein, Mitchell; Whitington, Peter F.; Jansen, Peter L M; Chowdhury, Namita Roy.

In: FASEB Journal, Vol. 6, No. 10, 07.1992, p. 2859-2863.

Research output: Contribution to journalArticle

Bosma, PJ, Roy-Chowdhury, J, Huang, TJ, Lahiri, P, Oude Elferink, RPJ, Van Es, HHG, Lederstein, M, Whitington, PF, Jansen, PLM & Chowdhury, NR 1992, 'Mechanisms of inherited deficiencies of multiple UDP-glucuronosyltransferase isoforms in two patients with Crigler-Najjar syndrome, type I', FASEB Journal, vol. 6, no. 10, pp. 2859-2863.
Bosma, Piter J. ; Roy-Chowdhury, Jayanta ; Huang, Tian Jun ; Lahiri, Pulak ; Oude Elferink, Ronald P J ; Van Es, Helmuth H G ; Lederstein, Mitchell ; Whitington, Peter F. ; Jansen, Peter L M ; Chowdhury, Namita Roy. / Mechanisms of inherited deficiencies of multiple UDP-glucuronosyltransferase isoforms in two patients with Crigler-Najjar syndrome, type I. In: FASEB Journal. 1992 ; Vol. 6, No. 10. pp. 2859-2863.
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abstract = "Crigler-Najjar syndrome, type I (CN-I) is a potentially lethal disorder characterized by severe unconjugated hyperbilirubinemia resulting from a recessively inherited deficiency of hepatic UDP-glucuronosyl-transferase (UGT) activity toward bilirubin (B-UGT). Two forms of B-UGT exist in human liver. mRNAs for these two forms and that for another isoform with activity toward simple phenols (P-UGT) have unique 5′ regions, but their 3′ regions are identical. The three mRNA species are derived from a single locus; the unique 5′ regions are encoded by single unique exons and the identical 3′ regions consist of four consecutive exons that are shared by all three isoforms. In this paper, we determined genetic lesions in two CN-I patients with deficiency of hepatic B-UGT and P-UGT activities. In one patient, there was a C→T substitution in exon 4 (common region) predicting the substitution of a serine residue with a phenylalanine residue; this mutation was present in the identical region of B-UGT and P-UGT mRNAs. In the other patient, a C→T substitution in exon 2 (common region) of the B-UGT/P-UGT locus resulted in a premature stop codon. This exon (132 nt) was absent in heptic B-UGT and P-UGT mRNAs of this patient due to exon skipping during pre-mRNA processing. Sequence abnormality of three distinct mRNA species explains the abnormality of multiple UGT isoforms in these patients. Presence of identical abnormalities in the common regions of the three mRNAs is consistent with the finding that the common 3′ regions of the two B-UGT mRNAs and the P-UGT mRNA are encoded by four shared exons.",
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AU - Van Es, Helmuth H G

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N2 - Crigler-Najjar syndrome, type I (CN-I) is a potentially lethal disorder characterized by severe unconjugated hyperbilirubinemia resulting from a recessively inherited deficiency of hepatic UDP-glucuronosyl-transferase (UGT) activity toward bilirubin (B-UGT). Two forms of B-UGT exist in human liver. mRNAs for these two forms and that for another isoform with activity toward simple phenols (P-UGT) have unique 5′ regions, but their 3′ regions are identical. The three mRNA species are derived from a single locus; the unique 5′ regions are encoded by single unique exons and the identical 3′ regions consist of four consecutive exons that are shared by all three isoforms. In this paper, we determined genetic lesions in two CN-I patients with deficiency of hepatic B-UGT and P-UGT activities. In one patient, there was a C→T substitution in exon 4 (common region) predicting the substitution of a serine residue with a phenylalanine residue; this mutation was present in the identical region of B-UGT and P-UGT mRNAs. In the other patient, a C→T substitution in exon 2 (common region) of the B-UGT/P-UGT locus resulted in a premature stop codon. This exon (132 nt) was absent in heptic B-UGT and P-UGT mRNAs of this patient due to exon skipping during pre-mRNA processing. Sequence abnormality of three distinct mRNA species explains the abnormality of multiple UGT isoforms in these patients. Presence of identical abnormalities in the common regions of the three mRNAs is consistent with the finding that the common 3′ regions of the two B-UGT mRNAs and the P-UGT mRNA are encoded by four shared exons.

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