Mechanism of class switching

B. K. Birshtein, D. R. Katzenberg, A. Weinreb, S. Giannini, J. Bard, C. F. Calvo, N. Martinez

Research output: Contribution to journalArticle

Abstract

We have documented the action of unequal sister chromatid exchange on antibody genes, and our studies provide the first molecular description of an unequal sister chromatid exchange event. We delineated the sequences that mediate class switch recombination in normal B cells through genomic Southern analysis of five IgG2-producing hybridomas. Successive subclass switching was documented in two of eight maps and may possibly account for interposed DNA in three additional maps, implying that subclass switching may be a relatively frequent occurrence in normal B cells. As in most other myelomas and hybridomas, the 5' breakpoint of switch recombination in our panel of hybridomas falls 5' to Cμ-associated tandem repeats, whereas the 3' breakpoint falls within Cγ-associated tandem repeats. We consider various hypotheses in the text, the most interesting of which is that deletion of Cμ-associated tandem repeats is a necessary first step before class switch recombination in mature B cells. This hypothesis suggests that Cμ-associated tandem repeats may be a separately regulated DNA element.

Original languageEnglish (US)
Pages (from-to)34-42
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume546
StatePublished - 1988

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Immunoglobulin Class Switching
Tandem Repeat Sequences
Hybridomas
Genetic Recombination
Sister Chromatid Exchange
B-Lymphocytes
Cells
Switches
DNA
Immunoglobulin G
Genes
Repeats
Antibodies
Recombination
Sister

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Birshtein, B. K., Katzenberg, D. R., Weinreb, A., Giannini, S., Bard, J., Calvo, C. F., & Martinez, N. (1988). Mechanism of class switching. Annals of the New York Academy of Sciences, 546, 34-42.

Mechanism of class switching. / Birshtein, B. K.; Katzenberg, D. R.; Weinreb, A.; Giannini, S.; Bard, J.; Calvo, C. F.; Martinez, N.

In: Annals of the New York Academy of Sciences, Vol. 546, 1988, p. 34-42.

Research output: Contribution to journalArticle

Birshtein, BK, Katzenberg, DR, Weinreb, A, Giannini, S, Bard, J, Calvo, CF & Martinez, N 1988, 'Mechanism of class switching', Annals of the New York Academy of Sciences, vol. 546, pp. 34-42.
Birshtein BK, Katzenberg DR, Weinreb A, Giannini S, Bard J, Calvo CF et al. Mechanism of class switching. Annals of the New York Academy of Sciences. 1988;546:34-42.
Birshtein, B. K. ; Katzenberg, D. R. ; Weinreb, A. ; Giannini, S. ; Bard, J. ; Calvo, C. F. ; Martinez, N. / Mechanism of class switching. In: Annals of the New York Academy of Sciences. 1988 ; Vol. 546. pp. 34-42.
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AU - Calvo, C. F.

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