Mechanism of bleomycin action: In vitro studies

Richard M. Burger; Jack Peisach; Susan Band Horwitz

doi:10.1016/0024-3205(81)90153-3

Mechanism of bleomycin action: In vitro studies

Richard M. Burger, Jack Peisach, Susan Band Horwitz

Molecular Pharmacology

Research output: Contribution to journal › Article › peer-review

127 Scopus citations

Abstract

The cytotoxic activity of bleomucin results from DNA cleavage, which is also accomplished in vitro by reaction mixtures containing Fe(II), drug and O₂. Bleomycin forms a complex with Fe(II) and O₂ in the presence or absence of DNA. The species attacking DNA forms rapidly from this complex. The nature of the attacking species and of the primary lesion(s) to DNA are not yet known, but two major insults to DNA have been characterized. They are the release of free bases from their glycosidic linkages and, at other residues, the cleavage of the polymer backbone at the deoxyribose C3-C4 bond.

Original language	English (US)
Pages (from-to)	715-727
Number of pages	13
Journal	Life Sciences
Volume	28
Issue number	7
DOIs	https://doi.org/10.1016/0024-3205(81)90153-3
State	Published - Feb 16 1981

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

Access to Document

10.1016/0024-3205(81)90153-3

Cite this

@article{d678bdf764d34da8aa6db431e9f8178c,

title = "Mechanism of bleomycin action: In vitro studies",

abstract = "The cytotoxic activity of bleomucin results from DNA cleavage, which is also accomplished in vitro by reaction mixtures containing Fe(II), drug and O2. Bleomycin forms a complex with Fe(II) and O2 in the presence or absence of DNA. The species attacking DNA forms rapidly from this complex. The nature of the attacking species and of the primary lesion(s) to DNA are not yet known, but two major insults to DNA have been characterized. They are the release of free bases from their glycosidic linkages and, at other residues, the cleavage of the polymer backbone at the deoxyribose C3-C4 bond.",

author = "Burger, {Richard M.} and Jack Peisach and {Band Horwitz}, Susan",

year = "1981",

month = feb,

day = "16",

doi = "10.1016/0024-3205(81)90153-3",

language = "English (US)",

volume = "28",

pages = "715--727",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "7",

}

TY - JOUR

T1 - Mechanism of bleomycin action

T2 - In vitro studies

AU - Burger, Richard M.

AU - Peisach, Jack

AU - Band Horwitz, Susan

PY - 1981/2/16

Y1 - 1981/2/16

N2 - The cytotoxic activity of bleomucin results from DNA cleavage, which is also accomplished in vitro by reaction mixtures containing Fe(II), drug and O2. Bleomycin forms a complex with Fe(II) and O2 in the presence or absence of DNA. The species attacking DNA forms rapidly from this complex. The nature of the attacking species and of the primary lesion(s) to DNA are not yet known, but two major insults to DNA have been characterized. They are the release of free bases from their glycosidic linkages and, at other residues, the cleavage of the polymer backbone at the deoxyribose C3-C4 bond.

AB - The cytotoxic activity of bleomucin results from DNA cleavage, which is also accomplished in vitro by reaction mixtures containing Fe(II), drug and O2. Bleomycin forms a complex with Fe(II) and O2 in the presence or absence of DNA. The species attacking DNA forms rapidly from this complex. The nature of the attacking species and of the primary lesion(s) to DNA are not yet known, but two major insults to DNA have been characterized. They are the release of free bases from their glycosidic linkages and, at other residues, the cleavage of the polymer backbone at the deoxyribose C3-C4 bond.

UR - http://www.scopus.com/inward/record.url?scp=0019467854&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019467854&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(81)90153-3

DO - 10.1016/0024-3205(81)90153-3

M3 - Article

C2 - 6164898

AN - SCOPUS:0019467854

SN - 0024-3205

VL - 28

SP - 715

EP - 727

JO - Life Sciences

JF - Life Sciences

IS - 7

ER -

Mechanism of bleomycin action: In vitro studies

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this