Mechanism-based affinity capture of sirtuins

Yana Cen, Jessica N. Falco, Ping Xu, Dou Yeon Youn, Anthony A. Sauve

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The ability to probe for catalytic activities of enzymes and to detect their abundance in complex biochemical contexts has traditionally relied on a combination of kinetic assays and techniques such as western blots that use expensive reagents such as antibodies. The ability to simultaneously detect activity and isolate a protein catalyst from a mixture is even more difficult and currently impossible in most cases. In this manuscript we describe a chemical approach that achieves this goal for a unique family of enzymes called sirtuins using novel chemical tools, enabling rapid detection of activity and isolation of these protein catalysts. Sirtuin deacetylases are implicated in the regulation of many physiological functions including energy metabolism, DNA-damage response, and cellular stress resistance. We synthesized an aminooxy-derivatized NAD+ and a pan-sirtuin inhibitor that reacts on sirtuin active sites to form a chemically stable complex that can subsequently be crosslinked to an aldehyde-substituted biotin. Subsequent retrieval of the biotinylated sirtuin complexes on streptavidin beads followed by gel electrophoresis enabled simultaneous detection of active sirtuins, isolation and molecular weight determination. We show that these tools are cross reactive against a variety of human sirtuin isoforms including SIRT1, SIRT2, SIRT3, SIRT5, SIRT6 and can react with microbial derived sirtuins as well. Finally, we demonstrate the ability to simultaneously detect multiple sirtuin isoforms in reaction mixtures with this methodology, establishing proof of concept tools for chemical studies of sirtuins in complex biological samples.

Original languageEnglish (US)
Pages (from-to)987-993
Number of pages7
JournalOrganic and Biomolecular Chemistry
Volume9
Issue number4
DOIs
StatePublished - Feb 21 2011
Externally publishedYes

Fingerprint

Sirtuins
affinity
enzymes
isolation
proteins
catalysts
Protein Isoforms
biotin
Chemical Phenomena
metabolism
electrophoresis
antibodies
aldehydes
beads
inhibitors
Catalysts
retrieval
Streptavidin
reagents
catalytic activity

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Mechanism-based affinity capture of sirtuins. / Cen, Yana; Falco, Jessica N.; Xu, Ping; Youn, Dou Yeon; Sauve, Anthony A.

In: Organic and Biomolecular Chemistry, Vol. 9, No. 4, 21.02.2011, p. 987-993.

Research output: Contribution to journalArticle

Cen, Yana ; Falco, Jessica N. ; Xu, Ping ; Youn, Dou Yeon ; Sauve, Anthony A. / Mechanism-based affinity capture of sirtuins. In: Organic and Biomolecular Chemistry. 2011 ; Vol. 9, No. 4. pp. 987-993.
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