Mechanism and regulation of mycobactin fatty acyl-AMP ligase FadD33

Olivia Vergnolle; Hua Xu; John S. Blanchard

doi:10.1074/jbc.M113.495549

Mechanism and regulation of mycobactin fatty acyl-AMP ligase FadD33

Olivia Vergnolle, Hua Xu, John S. Blanchard

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Background: Fatty acyl-AMP ligase FadD33 is required for mycobactin biosynthesis. Results: FadD33 catalyzes a two-step kinetic mechanism, and FadD33 activity is regulated by post-translational acetylation. Conclusion: Mycobactin FadD33 activity is reversibly regulated by Pat (acetylation) and DAc1 (deacetylation). Significant: Post-translational regulation via acetylation of enzymes can modulate siderophore biosynthesis.

Original language	English (US)
Pages (from-to)	28116-28125
Number of pages	10
Journal	Journal of Biological Chemistry
Volume	288
Issue number	39
DOIs	https://doi.org/10.1074/jbc.M113.495549
State	Published - Sep 27 2013
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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title = "Mechanism and regulation of mycobactin fatty acyl-AMP ligase FadD33",

abstract = "Background: Fatty acyl-AMP ligase FadD33 is required for mycobactin biosynthesis. Results: FadD33 catalyzes a two-step kinetic mechanism, and FadD33 activity is regulated by post-translational acetylation. Conclusion: Mycobactin FadD33 activity is reversibly regulated by Pat (acetylation) and DAc1 (deacetylation). Significant: Post-translational regulation via acetylation of enzymes can modulate siderophore biosynthesis.",

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AU - Xu, Hua

AU - Blanchard, John S.

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Y1 - 2013/9/27

N2 - Background: Fatty acyl-AMP ligase FadD33 is required for mycobactin biosynthesis. Results: FadD33 catalyzes a two-step kinetic mechanism, and FadD33 activity is regulated by post-translational acetylation. Conclusion: Mycobactin FadD33 activity is reversibly regulated by Pat (acetylation) and DAc1 (deacetylation). Significant: Post-translational regulation via acetylation of enzymes can modulate siderophore biosynthesis.

AB - Background: Fatty acyl-AMP ligase FadD33 is required for mycobactin biosynthesis. Results: FadD33 catalyzes a two-step kinetic mechanism, and FadD33 activity is regulated by post-translational acetylation. Conclusion: Mycobactin FadD33 activity is reversibly regulated by Pat (acetylation) and DAc1 (deacetylation). Significant: Post-translational regulation via acetylation of enzymes can modulate siderophore biosynthesis.

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Mechanism and regulation of mycobactin fatty acyl-AMP ligase FadD33

Abstract

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