Mechanism and regulation of mycobactin fatty acyl-AMP ligase FadD33

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: Fatty acyl-AMP ligase FadD33 is required for mycobactin biosynthesis. Results: FadD33 catalyzes a two-step kinetic mechanism, and FadD33 activity is regulated by post-translational acetylation. Conclusion: Mycobactin FadD33 activity is reversibly regulated by Pat (acetylation) and DAc1 (deacetylation). Significant: Post-translational regulation via acetylation of enzymes can modulate siderophore biosynthesis.

Original languageEnglish (US)
Pages (from-to)28116-28125
Number of pages10
JournalJournal of Biological Chemistry
Volume288
Issue number39
DOIs
StatePublished - Sep 27 2013

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Acetylation
Adenosine Monophosphate
Ligases
Biosynthesis
Siderophores
Thermodynamic properties
Kinetics
Enzymes
mycobactins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Mechanism and regulation of mycobactin fatty acyl-AMP ligase FadD33. / Vergnolle, Olivia M.; Xu, Hua; Blanchard, John S.

In: Journal of Biological Chemistry, Vol. 288, No. 39, 27.09.2013, p. 28116-28125.

Research output: Contribution to journalArticle

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