Measurement of macrophage growth and differentiation

Violeta Chitu, Yee Guide Yeung, Wenfeng Yu, Sayan Nandi, E. Richard Stanley

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

This unit provides protocols for measuring the abundance and growth of macrophage precursors in agar cultures and the proliferation of isolated mature macrophages in vitro, by either direct cell counting or by DNA measurement. Methods for the immunohisto-chemical identification of macrophages and the determination of their proliferative status in vivo by immunofluorescence are also included. It also describes methods for characterization of macrophage differentiation through the immunofluorescence analysis of cell-surface expression of CSF-1 receptor. Curr. Protoc. Immunol. 92:14.20.1-14.20.26.

Original languageEnglish (US)
Article number14.20
JournalCurrent Protocols in Immunology
Issue numberSUPPL.92
DOIs
StatePublished - Feb 2011

Keywords

  • CFU-M assay
  • CSF-1 receptor
  • Differentiation
  • Macrophage surface markers
  • Macrophages
  • Microglia
  • Myeloid

ASJC Scopus subject areas

  • Immunology

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