Measurement of isoprostanes as markers of oxidative stress

Dejan Milatovic, Thomas J. Montine, Michael Aschner

Research output: Chapter in Book/Report/Conference proceedingChapter

31 Citations (Scopus)

Abstract

Oxidative stress results from an imbalance between production of reactive oxygen and nitrogen species (ROS and RNS, respectively) and endogenous antioxidant defense mechanisms. Increased generation of ROS/RNS is implicated in the pathogenesis of a variety of human diseases, including neurodegenerative disease, atherosclerosis, cancer, and aging. However, measuring oxidative stress in biological systems is complex and requires accurate quantification of either free radicals or damaged biomolecules. One method to quantify oxidative injury is to measure lipid peroxidation. Lipids are readily attacked by free radicals, resulting in the formation of a number of peroxidation products. F <inf>2</inf>-isoprostanes (F<inf>2</inf>-IsoPs) are one group of these compounds and they are derived by the free radical peroxidation of arachidonic acid (AA). The F<inf>2</inf>-IsoPs, prostaglandine F<inf>2</inf>-like compounds, provide an accurate measure of oxidative stress both in vitro and in vivo. This protocol details current methodology used to quantify these molecules using gas chromatography-mass spectrometry (GC/MS).

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages195-204
Number of pages10
Volume758
ISBN (Print)9781617791697
DOIs
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume758
ISSN (Print)10643745

Fingerprint

Isoprostanes
Free Radicals
Oxidative Stress
F2-Isoprostanes
Reactive Nitrogen Species
Arachidonic Acid
Neurodegenerative Diseases
Gas Chromatography-Mass Spectrometry
Lipid Peroxidation
Reactive Oxygen Species
Atherosclerosis
Antioxidants
Lipids
Wounds and Injuries
Neoplasms

Keywords

  • Lipid peroxidation
  • Oxidative damage
  • Reactive oxygen species

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Milatovic, D., Montine, T. J., & Aschner, M. (2011). Measurement of isoprostanes as markers of oxidative stress. In Methods in Molecular Biology (Vol. 758, pp. 195-204). (Methods in Molecular Biology; Vol. 758). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-170-3_13

Measurement of isoprostanes as markers of oxidative stress. / Milatovic, Dejan; Montine, Thomas J.; Aschner, Michael.

Methods in Molecular Biology. Vol. 758 Humana Press Inc., 2011. p. 195-204 (Methods in Molecular Biology; Vol. 758).

Research output: Chapter in Book/Report/Conference proceedingChapter

Milatovic, D, Montine, TJ & Aschner, M 2011, Measurement of isoprostanes as markers of oxidative stress. in Methods in Molecular Biology. vol. 758, Methods in Molecular Biology, vol. 758, Humana Press Inc., pp. 195-204. https://doi.org/10.1007/978-1-61779-170-3_13
Milatovic D, Montine TJ, Aschner M. Measurement of isoprostanes as markers of oxidative stress. In Methods in Molecular Biology. Vol. 758. Humana Press Inc. 2011. p. 195-204. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-170-3_13
Milatovic, Dejan ; Montine, Thomas J. ; Aschner, Michael. / Measurement of isoprostanes as markers of oxidative stress. Methods in Molecular Biology. Vol. 758 Humana Press Inc., 2011. pp. 195-204 (Methods in Molecular Biology).
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