Measurement of β-hydroxybutyrate in acute hyperketonemia in human brain

J. W. Pan, F. W. Telang, J. H. Lee, R. A. De Graaf, D. L. Rothman, Daniel T. Stein, H. P. Hetherington

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We report the measurement of D-β-hydroxybutyrate (BHB) in the brains of six normal adult subjects during acute infusions of BHB. We used high field in vivo 1H magnetic resonance (MR) spectroscopy in the occipital lobe in conjunction with an acute infusion protocol to elevate plasma BHB levels from overnight fasted levels (0.20 ± 0.10 mM) to a steady state value of 2.12 ± 0.30 mM. At this level of hyperketonemia, we determined a tissue BHB level of 0.24 ± 0.04 mM. No increases in brain lactate levels were seen in these data. The concentrations of BHB and lactate were both considerably lower in comparison with previous data acquired in fasted adult subjects. This suggests that up-regulation of the monocarboxylic acid transporter occurs with fasting.

Original languageEnglish (US)
Pages (from-to)539-544
Number of pages6
JournalJournal of Neurochemistry
Volume79
Issue number3
DOIs
StatePublished - 2001

Fingerprint

Hydroxybutyrates
Lactic Acid
Brain
Monocarboxylic Acid Transporters
Magnetic resonance spectroscopy
Occipital Lobe
Fasting
Up-Regulation
Magnetic Resonance Spectroscopy
Tissue
Plasmas

Keywords

  • β-hydroxybutyrate
  • Brain
  • Ketone body
  • Lactate
  • Magnetic resonance spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Pan, J. W., Telang, F. W., Lee, J. H., De Graaf, R. A., Rothman, D. L., Stein, D. T., & Hetherington, H. P. (2001). Measurement of β-hydroxybutyrate in acute hyperketonemia in human brain. Journal of Neurochemistry, 79(3), 539-544. https://doi.org/10.1046/j.1471-4159.2001.00575.x

Measurement of β-hydroxybutyrate in acute hyperketonemia in human brain. / Pan, J. W.; Telang, F. W.; Lee, J. H.; De Graaf, R. A.; Rothman, D. L.; Stein, Daniel T.; Hetherington, H. P.

In: Journal of Neurochemistry, Vol. 79, No. 3, 2001, p. 539-544.

Research output: Contribution to journalArticle

Pan, JW, Telang, FW, Lee, JH, De Graaf, RA, Rothman, DL, Stein, DT & Hetherington, HP 2001, 'Measurement of β-hydroxybutyrate in acute hyperketonemia in human brain', Journal of Neurochemistry, vol. 79, no. 3, pp. 539-544. https://doi.org/10.1046/j.1471-4159.2001.00575.x
Pan, J. W. ; Telang, F. W. ; Lee, J. H. ; De Graaf, R. A. ; Rothman, D. L. ; Stein, Daniel T. ; Hetherington, H. P. / Measurement of β-hydroxybutyrate in acute hyperketonemia in human brain. In: Journal of Neurochemistry. 2001 ; Vol. 79, No. 3. pp. 539-544.
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