Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA

Roy M. Long; Robert H. Singer; Xiuhua Meng; Isabel Gonzalez; Kim Nasmyth; Ralf Peter Jansen

doi:10.1126/science.277.5324.383

Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA

Roy M. Long, Robert H. Singer, Xiuhua Meng, Isabel Gonzalez, Kim Nasmyth, Ralf Peter Jansen

Research output: Contribution to journal › Article › peer-review

424 Scopus citations

Abstract

Cell divisions that produce progeny differing in their patterns of gene expression are key to the development of multicellular organisms. In the budding yeast Saccharomyces cerevisiae, mother cells but not daughter cells can switch mating type because they selectively express the HO endonuclease gene. This asymmetry is due to the preferential accumulation of an unstable transcriptional repressor protein, Ash1p, in daughter cell nucleii. Here it is shown that ASH1 messenger RNA (mRNA) preferentially accumulates in daughter cells by a process that is dependent on actin and myosin. A cis- acting element in the 3'-untranslated region of ASH1 mRNA is sufficient to localize a chimeric RNA to daughter cells. These results suggest that localization of mRNA may have been an early property of the eukaryotic lineage.

Original language	English (US)
Pages (from-to)	383-387
Number of pages	5
Journal	Science
Volume	277
Issue number	5324
DOIs	https://doi.org/10.1126/science.277.5324.383
State	Published - Jul 18 1997
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1126/science.277.5324.383

Cite this

@article{4e1e3733af74436bb95f27f0f6616618,

title = "Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA",

abstract = "Cell divisions that produce progeny differing in their patterns of gene expression are key to the development of multicellular organisms. In the budding yeast Saccharomyces cerevisiae, mother cells but not daughter cells can switch mating type because they selectively express the HO endonuclease gene. This asymmetry is due to the preferential accumulation of an unstable transcriptional repressor protein, Ash1p, in daughter cell nucleii. Here it is shown that ASH1 messenger RNA (mRNA) preferentially accumulates in daughter cells by a process that is dependent on actin and myosin. A cis- acting element in the 3'-untranslated region of ASH1 mRNA is sufficient to localize a chimeric RNA to daughter cells. These results suggest that localization of mRNA may have been an early property of the eukaryotic lineage.",

author = "Long, {Roy M.} and Singer, {Robert H.} and Xiuhua Meng and Isabel Gonzalez and Kim Nasmyth and Jansen, {Ralf Peter}",

year = "1997",

month = jul,

day = "18",

doi = "10.1126/science.277.5324.383",

language = "English (US)",

volume = "277",

pages = "383--387",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5324",

}

TY - JOUR

T1 - Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA

AU - Long, Roy M.

AU - Singer, Robert H.

AU - Meng, Xiuhua

AU - Gonzalez, Isabel

AU - Nasmyth, Kim

AU - Jansen, Ralf Peter

PY - 1997/7/18

Y1 - 1997/7/18

N2 - Cell divisions that produce progeny differing in their patterns of gene expression are key to the development of multicellular organisms. In the budding yeast Saccharomyces cerevisiae, mother cells but not daughter cells can switch mating type because they selectively express the HO endonuclease gene. This asymmetry is due to the preferential accumulation of an unstable transcriptional repressor protein, Ash1p, in daughter cell nucleii. Here it is shown that ASH1 messenger RNA (mRNA) preferentially accumulates in daughter cells by a process that is dependent on actin and myosin. A cis- acting element in the 3'-untranslated region of ASH1 mRNA is sufficient to localize a chimeric RNA to daughter cells. These results suggest that localization of mRNA may have been an early property of the eukaryotic lineage.

AB - Cell divisions that produce progeny differing in their patterns of gene expression are key to the development of multicellular organisms. In the budding yeast Saccharomyces cerevisiae, mother cells but not daughter cells can switch mating type because they selectively express the HO endonuclease gene. This asymmetry is due to the preferential accumulation of an unstable transcriptional repressor protein, Ash1p, in daughter cell nucleii. Here it is shown that ASH1 messenger RNA (mRNA) preferentially accumulates in daughter cells by a process that is dependent on actin and myosin. A cis- acting element in the 3'-untranslated region of ASH1 mRNA is sufficient to localize a chimeric RNA to daughter cells. These results suggest that localization of mRNA may have been an early property of the eukaryotic lineage.

UR - http://www.scopus.com/inward/record.url?scp=0030875775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030875775&partnerID=8YFLogxK

U2 - 10.1126/science.277.5324.383

DO - 10.1126/science.277.5324.383

M3 - Article

C2 - 9219698

AN - SCOPUS:0030875775

SN - 0036-8075

VL - 277

SP - 383

EP - 387

JO - Science

JF - Science

IS - 5324

ER -

Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this