Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA

Roy M. Long, Robert H. Singer, Xiuhua Meng, Isabel Gonzalez, Kim Nasmyth, Ralf Peter Jansen

Research output: Contribution to journalArticle

373 Citations (Scopus)

Abstract

Cell divisions that produce progeny differing in their patterns of gene expression are key to the development of multicellular organisms. In the budding yeast Saccharomyces cerevisiae, mother cells but not daughter cells can switch mating type because they selectively express the HO endonuclease gene. This asymmetry is due to the preferential accumulation of an unstable transcriptional repressor protein, Ash1p, in daughter cell nucleii. Here it is shown that ASH1 messenger RNA (mRNA) preferentially accumulates in daughter cells by a process that is dependent on actin and myosin. A cis- acting element in the 3'-untranslated region of ASH1 mRNA is sufficient to localize a chimeric RNA to daughter cells. These results suggest that localization of mRNA may have been an early property of the eukaryotic lineage.

Original languageEnglish (US)
Pages (from-to)383-387
Number of pages5
JournalScience
Volume277
Issue number5324
DOIs
StatePublished - Jul 18 1997
Externally publishedYes

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Yeasts
Messenger RNA
Repressor Proteins
Saccharomycetales
Endonucleases
3' Untranslated Regions
Myosins
Cell Division
Saccharomyces cerevisiae
Actins
Stem Cells
RNA
Gene Expression
Genes

ASJC Scopus subject areas

  • General

Cite this

Long, R. M., Singer, R. H., Meng, X., Gonzalez, I., Nasmyth, K., & Jansen, R. P. (1997). Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA. Science, 277(5324), 383-387. https://doi.org/10.1126/science.277.5324.383

Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA. / Long, Roy M.; Singer, Robert H.; Meng, Xiuhua; Gonzalez, Isabel; Nasmyth, Kim; Jansen, Ralf Peter.

In: Science, Vol. 277, No. 5324, 18.07.1997, p. 383-387.

Research output: Contribution to journalArticle

Long, RM, Singer, RH, Meng, X, Gonzalez, I, Nasmyth, K & Jansen, RP 1997, 'Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA', Science, vol. 277, no. 5324, pp. 383-387. https://doi.org/10.1126/science.277.5324.383
Long, Roy M. ; Singer, Robert H. ; Meng, Xiuhua ; Gonzalez, Isabel ; Nasmyth, Kim ; Jansen, Ralf Peter. / Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA. In: Science. 1997 ; Vol. 277, No. 5324. pp. 383-387.
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