Maternal substrate utilization programs the development of the metabolic syndrome in male mice exposed to high fat in utero

Kirsten Hartil, Patricia M. Vuguin, Michael Kruse, Esther Schmuel, Ariana Fiallo, Carlos Vargas, Matthew J. Warner, Jorge L. Durand, Linda A. Jelicks, Maureen J. Charron

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Studies were conducted to determine whether maternal substrate utilization during pregnancy affects fetal growth and predisposes offspring to metabolic disease. Female wild-type (WT) and glucose transporter 4 heterozygous mice (G4±, a model of altered peripheral substrate utilization) were fed high-fat diet (HFD, 35.5% fat) or control chow (C, 9.5% fat) for 2 wk before mating, throughout pregnancy and lactation (IU/L). WT HFD females exhibited increased serum nonesterified fatty acid and lactate levels and increased hepatic mRNA expression of peroxisome proliferator-activated receptor γ coactivator-1-β and SREBP-1c, consistent with increased lipogenesis. G4± HFD females exhibited enhanced lipid clearance, and exposure to HFD did not increase hepatic gene expression. HFD independent of maternal genotype decreased fetal growth and birth weight. WT offspring were weaned onto a low-fat diet (5.6% fat). Male offspring of WT mothers exposed to HFD exhibited "catch-up" growth accompanied by increased adiposity, impaired glucose tolerance, and insulin sensitivity. In contrast, male offspring of G4± HFD mothers did not exhibit any characteristics of metabolic syndrome. These data suggest that differences in maternal substrate utilization influence offspring metabolic phenotype.

Original languageEnglish (US)
Pages (from-to)368-373
Number of pages6
JournalPediatric Research
Volume66
Issue number4
DOIs
StatePublished - 2009

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Program Development
Fats
Mothers
Fetal Development
Glucose Transporter Type 4
Sterol Regulatory Element Binding Protein 1
Pregnancy
Fat-Restricted Diet
Lipogenesis
Fetal Weight
Peroxisome Proliferator-Activated Receptors
Glucose Intolerance
Liver
Metabolic Diseases
Adiposity
High Fat Diet
Lactation
Nonesterified Fatty Acids
Birth Weight
Insulin Resistance

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Maternal substrate utilization programs the development of the metabolic syndrome in male mice exposed to high fat in utero. / Hartil, Kirsten; Vuguin, Patricia M.; Kruse, Michael; Schmuel, Esther; Fiallo, Ariana; Vargas, Carlos; Warner, Matthew J.; Durand, Jorge L.; Jelicks, Linda A.; Charron, Maureen J.

In: Pediatric Research, Vol. 66, No. 4, 2009, p. 368-373.

Research output: Contribution to journalArticle

Hartil, K, Vuguin, PM, Kruse, M, Schmuel, E, Fiallo, A, Vargas, C, Warner, MJ, Durand, JL, Jelicks, LA & Charron, MJ 2009, 'Maternal substrate utilization programs the development of the metabolic syndrome in male mice exposed to high fat in utero', Pediatric Research, vol. 66, no. 4, pp. 368-373. https://doi.org/10.1203/PDR.0b013e3181b33375
Hartil, Kirsten ; Vuguin, Patricia M. ; Kruse, Michael ; Schmuel, Esther ; Fiallo, Ariana ; Vargas, Carlos ; Warner, Matthew J. ; Durand, Jorge L. ; Jelicks, Linda A. ; Charron, Maureen J. / Maternal substrate utilization programs the development of the metabolic syndrome in male mice exposed to high fat in utero. In: Pediatric Research. 2009 ; Vol. 66, No. 4. pp. 368-373.
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