Maternal prenatal undernutrition programs adipose tissue gene expression in adult male rat offspring under high-fat diet

Marie Amélie Lukaszewski, Sylvain Mayeur, Isabelle Fajardy, Fabien Delahaye, Isabelle Dutriez-Casteloot, Valérie Montel, Anne Dickes-Coopman, Christine Laborie, Jean Lesage, Didier Vieau, Christophe Breton

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Several studies have shown that maternal undernutrition leading to low birth weight predisposes offspring to the development of metabolic pathologies such as obesity. Using a model of prenatal maternal 70% food restriction diet (FR30) in rat, we evaluated whether postweaning high-fat (HF) diet would amplify the phenotype observed under standard diet. We investigated biological parameters as well as gene expression profile focusing on white adipose tissues (WAT) of adult offspring. FR30 procedure does not worsen the metabolic syndrome features induced by HF diet. However, FR30HF rats displayed catch-up growth to match the body weight of adult control HF animals, suggesting an increase of adiposity while showing hyperleptinemia and a blunted increase of corticosterone. Using quantitative RT-PCR array, we demonstrated that FR30HF rats exhibited leptin and Ob-Rb as well as many peptide precursor and receptor gene expression variations in WAT. We also showed that the expression of genes involved in adipogenesis was modified in FR30HF animals in a depot-specific manner. We observed an opposite variation of STAT3 phosphorylation levels, suggesting that leptin sensitivity is modified in WAT adult FR30 offspring. We demonstrated that 11 β-HSD1, 11β-HSD2, GR, and MR genes are coexpressed in WAT and that FR30 procedure modifies gene expression levels, especially under HF diet. In particular, level variation of 11 β -HSD2, whose protein expression was detected by Western blotting, may represent a novel mechanism that may affect WAT glucocorticoid sensitivity. Data suggest that maternal undernutrition differently programs the adult offspring WAT gene expression profile that may predispose for altered fat deposition.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume301
Issue number3
DOIs
StatePublished - Sep 2011
Externally publishedYes

Fingerprint

White Adipose Tissue
High Fat Diet
Malnutrition
Adipose Tissue
Mothers
Gene Expression
Adult Children
Leptin
Transcriptome
Fats
Diet
Adipogenesis
Peptide Receptors
Adiposity
Low Birth Weight Infant
Corticosterone
Glucocorticoids
Obesity
Western Blotting
Body Weight

Keywords

  • Maternal undernutrition

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Endocrinology, Diabetes and Metabolism

Cite this

Maternal prenatal undernutrition programs adipose tissue gene expression in adult male rat offspring under high-fat diet. / Lukaszewski, Marie Amélie; Mayeur, Sylvain; Fajardy, Isabelle; Delahaye, Fabien; Dutriez-Casteloot, Isabelle; Montel, Valérie; Dickes-Coopman, Anne; Laborie, Christine; Lesage, Jean; Vieau, Didier; Breton, Christophe.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 301, No. 3, 09.2011.

Research output: Contribution to journalArticle

Lukaszewski, MA, Mayeur, S, Fajardy, I, Delahaye, F, Dutriez-Casteloot, I, Montel, V, Dickes-Coopman, A, Laborie, C, Lesage, J, Vieau, D & Breton, C 2011, 'Maternal prenatal undernutrition programs adipose tissue gene expression in adult male rat offspring under high-fat diet', American Journal of Physiology - Endocrinology and Metabolism, vol. 301, no. 3. https://doi.org/10.1152/ajpendo.00011.2011
Lukaszewski, Marie Amélie ; Mayeur, Sylvain ; Fajardy, Isabelle ; Delahaye, Fabien ; Dutriez-Casteloot, Isabelle ; Montel, Valérie ; Dickes-Coopman, Anne ; Laborie, Christine ; Lesage, Jean ; Vieau, Didier ; Breton, Christophe. / Maternal prenatal undernutrition programs adipose tissue gene expression in adult male rat offspring under high-fat diet. In: American Journal of Physiology - Endocrinology and Metabolism. 2011 ; Vol. 301, No. 3.
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