Maternal perinatal undernutrition drastically reduces postnatal leptin surge and affects the development of arcuate nucleus proopiomelanocortin neurons in neonatal male rat pups

Fabien Delahaye, Christophe Breton, Pierre Yves Risold, Mihaela Enache, Isabelle Dutriez-Casteloot, Christine Laborie, Jean Lesage, Didier Vieau

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

A growing body of evidence suggests that maternal undernutrition sensitizes the offspring to the development of energy balance metabolic disorders such as type 2 diabetes, dyslipidemia, and obesity. The present study aimed at examining the impact of maternal undernutrition on leptin plasma levels in newborn male rats and on the arcuate nucleus proopiomelanocortin (POMC) and neuropeptide Y (NPY) neurons that are major leptin targets. Using a model of perinatal maternal 50% food-restricted diet (FR50) in the rat, we evaluated leptin plasma levels and hypothalamic POMC and NPY gene expression from postnatal day (PND) 4 to PND30 in both control and FR50 offspring. In control rats, a postnatal peak of plasma leptin was observed between PND4 and PND14 that reached a maximal value at PND10 (5.17 ± 0.53 ng/ml), whereas it was dramatically reduced in FR50 pups with the higher concentration at PND7 (0.93 ± 0.23 ng/ml). In FR50 animals, using semiquantitative RT-PCR and in situ hybridization, we showed that the hypothalamic POMC mRNA level was decreased from PND14 until PND30, whereas NPY gene expression was not significantly modified. In PND21 FR50 animals, we observed strikingly reduced immunoreactive β-endorphin nerve fibers projecting to the hypothalamic paraventricular nucleus without affecting NPY projections. Our data showed that maternal undernutrition drastically reduces the postnatal surge of plasma leptin, disturbing particularly the hypothalamic wiring as well as the gene expression of the anorexigenic POMC neurons in male rat pups. These alterations might contribute to the adult metabolic disorders resulting from perinatal growth retardation.

Original languageEnglish (US)
Pages (from-to)470-475
Number of pages6
JournalEndocrinology
Volume149
Issue number2
DOIs
StatePublished - Feb 2008
Externally publishedYes

Fingerprint

Pro-Opiomelanocortin
Arcuate Nucleus of Hypothalamus
Leptin
Malnutrition
Neuropeptide Y
Mothers
Neurons
Gene Expression
Endorphins
Paraventricular Hypothalamic Nucleus
Dyslipidemias
Nerve Fibers
Type 2 Diabetes Mellitus
In Situ Hybridization
Obesity
Diet
Food
Polymerase Chain Reaction
Messenger RNA
Growth

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Maternal perinatal undernutrition drastically reduces postnatal leptin surge and affects the development of arcuate nucleus proopiomelanocortin neurons in neonatal male rat pups. / Delahaye, Fabien; Breton, Christophe; Risold, Pierre Yves; Enache, Mihaela; Dutriez-Casteloot, Isabelle; Laborie, Christine; Lesage, Jean; Vieau, Didier.

In: Endocrinology, Vol. 149, No. 2, 02.2008, p. 470-475.

Research output: Contribution to journalArticle

Delahaye, Fabien ; Breton, Christophe ; Risold, Pierre Yves ; Enache, Mihaela ; Dutriez-Casteloot, Isabelle ; Laborie, Christine ; Lesage, Jean ; Vieau, Didier. / Maternal perinatal undernutrition drastically reduces postnatal leptin surge and affects the development of arcuate nucleus proopiomelanocortin neurons in neonatal male rat pups. In: Endocrinology. 2008 ; Vol. 149, No. 2. pp. 470-475.
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