TY - JOUR
T1 - Maternal microchimerism in muscle biopsies from children with juvenile dermatomyositis
AU - Ye, Yi
AU - Van Zyl, Berendine
AU - Varsani, Hemlata
AU - Wedderburn, Lucy R.
AU - Ramanan, Athimalaipet
AU - Gillespie, Kathleen M.
N1 - Funding Information:
We are grateful to the families participating in the Juvenile Dermatomyositis Cohort Biomarker Study and Repository (UK and Ireland). The UK JDM Cohort and Biomarker Study is supported by grants from the Wellcome Trust UK, Action Medical Research UK and the Henry Smith Charity.
Funding Information:
Funding: This project was funded by a grant from North Bristol Trust. The development of the methodology described was funded by grants from Diabetes UK, the Juvenile Diabetes Research Foundation and the European Foundation for the Study of Diabetes.
PY - 2012/6
Y1 - 2012/6
N2 - Objective: Recent advances in molecular techniques have revealed that there is bi-directional transfer of cells between mother and child during pregnancy, and the presence of a mother's cells in her child has been termed maternal microchimerism (MMc). There is the potential for maternal cells to provoke inappropriate immune responses in the child, which could be a factor in autoimmunity including JDM. The aim of this study was to determine whether maternal (female) cells could be detected in frozen muscle sections from seven males (age range 3-13 years) with JDM participating in the Juvenile Dermatomyositis National (UK and Ireland) Cohort Biomarker Study and Repository for Idiopathic Inflammatory Myopathies and sections of muscle controls (age range 2-12 years). Methods: At least 1000 cells from each section underwent FISH and confocal imaging through each nucleus. Concomitant IF for CD45 was used to determine whether MMc in muscle were lymphocytes. A non-parametric Mann-Whitney U-test was used to detect statistical differences. Results: The frequency of MMc was higher in JDM muscle (0.42-1.14%) than in controls (0.08-0.42%) P = 0.01. No CD45 + MMc were observed. Conclusion: These data confirm an increased frequency of MMc in JDM. More detailed characterization of MMc is required, particularly using phenotypic markers, to explain the role of these cells in JDM.
AB - Objective: Recent advances in molecular techniques have revealed that there is bi-directional transfer of cells between mother and child during pregnancy, and the presence of a mother's cells in her child has been termed maternal microchimerism (MMc). There is the potential for maternal cells to provoke inappropriate immune responses in the child, which could be a factor in autoimmunity including JDM. The aim of this study was to determine whether maternal (female) cells could be detected in frozen muscle sections from seven males (age range 3-13 years) with JDM participating in the Juvenile Dermatomyositis National (UK and Ireland) Cohort Biomarker Study and Repository for Idiopathic Inflammatory Myopathies and sections of muscle controls (age range 2-12 years). Methods: At least 1000 cells from each section underwent FISH and confocal imaging through each nucleus. Concomitant IF for CD45 was used to determine whether MMc in muscle were lymphocytes. A non-parametric Mann-Whitney U-test was used to detect statistical differences. Results: The frequency of MMc was higher in JDM muscle (0.42-1.14%) than in controls (0.08-0.42%) P = 0.01. No CD45 + MMc were observed. Conclusion: These data confirm an increased frequency of MMc in JDM. More detailed characterization of MMc is required, particularly using phenotypic markers, to explain the role of these cells in JDM.
KW - Autoimmunity
KW - Juvenile dermatomyositis
KW - Maternal microchimersim
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U2 - 10.1093/rheumatology/ker430
DO - 10.1093/rheumatology/ker430
M3 - Article
C2 - 22271755
AN - SCOPUS:84861475874
SN - 1462-0324
VL - 51
SP - 987
EP - 991
JO - Rheumatology (United Kingdom)
JF - Rheumatology (United Kingdom)
IS - 6
M1 - ker430
ER -