Mast cells and macrophages in normal C57/BL/6 mice

Christine Gersch, Oliver Dewald, Martin Zoerlein, Lloyd H. Michael, Mark L. Entman, Nikolaos G. Frangogiannis

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Mast cells and macrophages have an important role in immunity and inflammation. Because mice are used extensively for experimental studies investigating immunological and inflammatory responses, we examined mast cell and macrophage distribution in normal murine tissues. Mast cells were abundant in the murine dermis, tongue, and skeletal muscle but were rarely found in the heart, lung, spleen, kidney, liver, and the bowel mucosa. In contrast, dogs exhibited large numbers of mast cells in the lung parenchyma, liver, and bowel. Some murine dermal mast cells had long cytoplasmic projections filled with granular content. Mouse mast cells demonstrated intense histamine immunoreactivity and were identified with histochemical enzymatic techniques for tryptase and chymase. Macrophages, identified using the monoclonal antibody F4/80, were abundant in the spleen, lung, liver, kidney, and bowel but relatively rare in the heart, tongue, and dermis. Using a nuclease protection assay we investigated mRNA expression of stem cell factor (SCF), a crucial survival factor for mast cells, and the macrophage growth factors macrophage colony stimulating factor (M-CSF) and granulocyte macrophage colony stimulating factor (GM-CSF). Stem cell factor mRNA was highly expressed in the murine lung. Relatively low levels of SCF mRNA expression were found in the tongue and earlobe, which are tissues containing a high number of mast cells. Macrophage CSF and GM-CSF mRNA was highly expressed in the lung and spleen. The murine heart, an organ with a low macrophage content, expressed high levels of M-CSF but negligible levels of GM-CSF mRNA. Constitutive growth factor mRNA expression in murine tissues without significant populations of mast cells and macrophages may suggest an alternative role for these factors in tissue homeostasis.

Original languageEnglish (US)
Pages (from-to)41-49
Number of pages9
JournalHistochemistry and Cell Biology
Volume118
Issue number1
StatePublished - 2002
Externally publishedYes

Fingerprint

macrophages
Macrophages
Mast Cells
mice
Stem Cell Factor
Messenger RNA
Granulocyte-Macrophage Colony-Stimulating Factor
Lung
lungs
Tongue
spleen
Macrophage Colony-Stimulating Factor
tongue
Spleen
stem cells
Dermis
Stem cells
liver
Liver
Nuclease Protection Assays

Keywords

  • Growth factors
  • Macrophage
  • Mast cell
  • Mouse
  • Tryptase

ASJC Scopus subject areas

  • Cell Biology
  • Instrumentation

Cite this

Gersch, C., Dewald, O., Zoerlein, M., Michael, L. H., Entman, M. L., & Frangogiannis, N. G. (2002). Mast cells and macrophages in normal C57/BL/6 mice. Histochemistry and Cell Biology, 118(1), 41-49.

Mast cells and macrophages in normal C57/BL/6 mice. / Gersch, Christine; Dewald, Oliver; Zoerlein, Martin; Michael, Lloyd H.; Entman, Mark L.; Frangogiannis, Nikolaos G.

In: Histochemistry and Cell Biology, Vol. 118, No. 1, 2002, p. 41-49.

Research output: Contribution to journalArticle

Gersch, C, Dewald, O, Zoerlein, M, Michael, LH, Entman, ML & Frangogiannis, NG 2002, 'Mast cells and macrophages in normal C57/BL/6 mice', Histochemistry and Cell Biology, vol. 118, no. 1, pp. 41-49.
Gersch C, Dewald O, Zoerlein M, Michael LH, Entman ML, Frangogiannis NG. Mast cells and macrophages in normal C57/BL/6 mice. Histochemistry and Cell Biology. 2002;118(1):41-49.
Gersch, Christine ; Dewald, Oliver ; Zoerlein, Martin ; Michael, Lloyd H. ; Entman, Mark L. ; Frangogiannis, Nikolaos G. / Mast cells and macrophages in normal C57/BL/6 mice. In: Histochemistry and Cell Biology. 2002 ; Vol. 118, No. 1. pp. 41-49.
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