Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR)

David S. Burz, Kaushik Dutta, David Cowburn, Alexander Shekhtman

Research output: Contribution to journalArticle

126 Scopus citations

Abstract

We describe a high-throughput in-cell nuclear magnetic resonance (NMR)-based method for mapping the structural changes that accompany protein-protein interactions (STINT-NMR). The method entails sequentially expressing two (or more) proteins within a single bacterial cell in a time-controlled manner and monitoring the protein interactions using in-cell NMR spectroscopy. The resulting spectra provide a complete titration of the interaction and define structural details of the interacting surfaces at atomic resolution.

Original languageEnglish (US)
Pages (from-to)91-93
Number of pages3
JournalNature Methods
Volume3
Issue number2
DOIs
StatePublished - Feb 1 2006

    Fingerprint

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this