Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR)

David S. Burz, Kaushik Dutta, David Cowburn, Alexander Shekhtman

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

We describe a high-throughput in-cell nuclear magnetic resonance (NMR)-based method for mapping the structural changes that accompany protein-protein interactions (STINT-NMR). The method entails sequentially expressing two (or more) proteins within a single bacterial cell in a time-controlled manner and monitoring the protein interactions using in-cell NMR spectroscopy. The resulting spectra provide a complete titration of the interaction and define structural details of the interacting surfaces at atomic resolution.

Original languageEnglish (US)
Pages (from-to)91-93
Number of pages3
JournalNature Methods
Volume3
Issue number2
DOIs
StatePublished - Feb 2006

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Magnetic Resonance Spectroscopy
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

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Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR). / Burz, David S.; Dutta, Kaushik; Cowburn, David; Shekhtman, Alexander.

In: Nature Methods, Vol. 3, No. 2, 02.2006, p. 91-93.

Research output: Contribution to journalArticle

Burz, David S. ; Dutta, Kaushik ; Cowburn, David ; Shekhtman, Alexander. / Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR). In: Nature Methods. 2006 ; Vol. 3, No. 2. pp. 91-93.
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