Mapping of 34 minisatellite loci resolved by two-dimensional DNA typing

Anders Dupont Børglum, M. Nyegaard, A. B. Kvistgaard, E. Mullaart, A. G. Uitterlinden, Jan Vijg, T. A. Kruse

Research output: Contribution to journalArticle

Abstract

Two-dimensional (2-D) DNA typing is based on electrophoretic separation of genomic DNA fragments in two dimensions according to independent criteria (size and basepair sequence), followed by hybridization analysis using multilocus probes. The technique allows simultaneous visualization of several hundred loci as spots in a 2-D pattern. The majority of the loci resolved are polymorphic. Using linkage analysis in a large CEPH family, this study reports the mapping of 34 loci detected by the minisatellite core probe 33.6. By multipoint linkage analysis, regional chromosome positions of the 33.6 loci could be deduced, showing no evidence of clustering. In the analysis of spot patterns, use was made of a computerized image analysis system specifically designed for 2-D DNA typing. Since experimental variations between different separation patterns were automatically corrected for with this program, rapid and reliable scorings could be obtained. The results presented demonstrate the availability of reliable genetic information throughout the 2-D separation pattern. Adding the use of semiautomated computerized pattern analysis, this study further substantiates the applicability of 2-D DNA typing in genome scanning, not only in theoretical but also in practical terms. Moreover, it can be anticipated that this method will have a specific advantage in studies that scan for trinucleotide repeat expansions and somatic instability, where the repeat sequences detected by appropriate core probes are of particular interest.

Original languageEnglish (US)
Pages (from-to)248-256
Number of pages9
JournalCytogenetics and Cell Genetics
Volume79
Issue number3-4
StatePublished - 1997
Externally publishedYes

Fingerprint

Minisatellite Repeats
DNA Fingerprinting
Trinucleotide Repeat Expansion
Cluster Analysis
Chromosomes
Genome
DNA

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Børglum, A. D., Nyegaard, M., Kvistgaard, A. B., Mullaart, E., Uitterlinden, A. G., Vijg, J., & Kruse, T. A. (1997). Mapping of 34 minisatellite loci resolved by two-dimensional DNA typing. Cytogenetics and Cell Genetics, 79(3-4), 248-256.

Mapping of 34 minisatellite loci resolved by two-dimensional DNA typing. / Børglum, Anders Dupont; Nyegaard, M.; Kvistgaard, A. B.; Mullaart, E.; Uitterlinden, A. G.; Vijg, Jan; Kruse, T. A.

In: Cytogenetics and Cell Genetics, Vol. 79, No. 3-4, 1997, p. 248-256.

Research output: Contribution to journalArticle

Børglum, AD, Nyegaard, M, Kvistgaard, AB, Mullaart, E, Uitterlinden, AG, Vijg, J & Kruse, TA 1997, 'Mapping of 34 minisatellite loci resolved by two-dimensional DNA typing', Cytogenetics and Cell Genetics, vol. 79, no. 3-4, pp. 248-256.
Børglum AD, Nyegaard M, Kvistgaard AB, Mullaart E, Uitterlinden AG, Vijg J et al. Mapping of 34 minisatellite loci resolved by two-dimensional DNA typing. Cytogenetics and Cell Genetics. 1997;79(3-4):248-256.
Børglum, Anders Dupont ; Nyegaard, M. ; Kvistgaard, A. B. ; Mullaart, E. ; Uitterlinden, A. G. ; Vijg, Jan ; Kruse, T. A. / Mapping of 34 minisatellite loci resolved by two-dimensional DNA typing. In: Cytogenetics and Cell Genetics. 1997 ; Vol. 79, No. 3-4. pp. 248-256.
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