MAP kinase function in amoeboid chemotaxis

Yiwen Wang, Joey Liu, Jeffrey E. Segall

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Mutants lacking the MAP kinase DdERK2 show reduced chemotactic responses to folate and cAMP. Analysis of cAMP chemotaxis shows that Dderk2- cells are defective in chemotaxis to high concentrations of cAMP. This defect is due to an inability to repolarize in the continued presence of high concentrations of cAMP. Under these conditions, the speed of movement of mutant cells remains low. Instead of generating a leading pseudopod, mutant cells generate transient crown-like structures over multiple regions of the cell surface. These structures differ from pseudopods in that they contain myosin II as well as F actin and coronin. These studies identify a role for MAP kinases in coordinating the formation of cell projections generated in response to chemoattractants.

Original languageEnglish (US)
Pages (from-to)373-383
Number of pages11
JournalJournal of cell science
Volume111
Issue number3
StatePublished - Mar 16 1998

Keywords

  • Cell movement
  • Chemotaxis
  • Erk
  • Map kinase

ASJC Scopus subject areas

  • Cell Biology

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