Manganese Uptake and Efflux in Cultured Rat Astrocytes

Michael Aschner, Maureen Gannon, Harold K. Kimelberg

Research output: Contribution to journalArticle

163 Scopus citations

Abstract

Abstract: Astrocytes play a central role in manganese (Mn) regulation in the CNS. Using primary astrocyte cultures from neonatal rat brains, these studies demonstrate a specific highaffinity transport system for Mn2+. Saturation kinetics are clearly indicated by both l/s versus l/s plots (Km= 0.30 ± 0.03 μMM; Vmax= 0.30 ± 0.02 nmol/mg of protein/min) and plots of v versus [s]. Several divalent cations (Co2, Zn2+, and Pb2+) failed to inhibit the initial rate of 54Mn2+ uptake. In contrast, extracellular Ca2+ at 10 μM decreased 54Mn2+ uptake. Exchange with extracellular Mn2+ was not obligatory for the efflux of 54Mn2+ into extracellular medium because efflux occurred into Mn2+‐free extracellular medium, but efflux of 54Mn2+ was enhanced when astrocytes were equilibrated in the presence of unlabeled Mn2+. Efflux of 54Mn2+ was biphasic with both a rapid and a slow component. Efflux was most rapid during the first 10 min of incubation, with 27.5 ± 2.2% of 54Mn2+ transported extracellularly, and 37.2 ± 1.2% of preloaded 54Mn2+ was retained by the astrocytes at 120 min. These studies show, for the first time, that mammalian astrocytes can transport Mn via a specific transport system.

Original languageEnglish (US)
Pages (from-to)730-735
Number of pages6
JournalJournal of Neurochemistry
Volume58
Issue number2
DOIs
StatePublished - Feb 1992
Externally publishedYes

Keywords

  • Astrocytes
  • Efflux
  • Manganese
  • Rat
  • Tissue culture
  • Uptake

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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