Manganese transport by rat brain endothelial (RBE4) cell-based transwell model in the presence of astrocyte conditioned media

Vanessa A. Fitsanakis, Greg Piccola, Judy L. Aschner, Michael Aschner

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Manganese (Mn), an essential nutrient, is neurotoxic at high levels and has been associated with the development of a parkinsonian syndrome termed manganism. Currently, the mechanisms responsible for transporting Mn across the blood-brain barrier (BBB) are unknown. By using rat brain endothelial 4 (RBE4) cell monolayers cultured in astrocyte-conditioned media (ACM), we examine the effects of temperature, energy, proton (pH), iron (Fe), and sodium (Na +) dependence on Mn transport. Our results suggest that Mn transport is temperature, energy, and pH dependent, but not Fe or Na+ dependent. These data suggest that Mn transport across the BBB is an active process, but they also demonstrate that the presence of ACM in endothelial cell cultures decreases the permeability of these cells to Mn, reinforcing the use of ACM or astrocyte cocultures in studies examining metal transport across the BBB.

Original languageEnglish (US)
Pages (from-to)235-243
Number of pages9
JournalJournal of Neuroscience Research
Volume81
Issue number2
DOIs
StatePublished - Jul 15 2005
Externally publishedYes

Fingerprint

Manganese
Conditioned Culture Medium
Astrocytes
Endothelial Cells
Brain
Blood-Brain Barrier
Temperature
Parkinsonian Disorders
Coculture Techniques
Protons
Permeability
Iron
Cell Culture Techniques
Metals
Sodium
Food

Keywords

  • Astrocyte-conditioned medium
  • Energy dependence
  • Iron dependence
  • Proton dependence
  • Sodium dependence

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Manganese transport by rat brain endothelial (RBE4) cell-based transwell model in the presence of astrocyte conditioned media. / Fitsanakis, Vanessa A.; Piccola, Greg; Aschner, Judy L.; Aschner, Michael.

In: Journal of Neuroscience Research, Vol. 81, No. 2, 15.07.2005, p. 235-243.

Research output: Contribution to journalArticle

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