Manganese transport by rat brain endothelial (RBE4) cell-based transwell model in the presence of astrocyte conditioned media

Vanessa A. Fitsanakis, Greg Piccola, Judy L. Aschner, Michael Aschner

Research output: Contribution to journalArticle

16 Scopus citations


Manganese (Mn), an essential nutrient, is neurotoxic at high levels and has been associated with the development of a parkinsonian syndrome termed manganism. Currently, the mechanisms responsible for transporting Mn across the blood-brain barrier (BBB) are unknown. By using rat brain endothelial 4 (RBE4) cell monolayers cultured in astrocyte-conditioned media (ACM), we examine the effects of temperature, energy, proton (pH), iron (Fe), and sodium (Na +) dependence on Mn transport. Our results suggest that Mn transport is temperature, energy, and pH dependent, but not Fe or Na+ dependent. These data suggest that Mn transport across the BBB is an active process, but they also demonstrate that the presence of ACM in endothelial cell cultures decreases the permeability of these cells to Mn, reinforcing the use of ACM or astrocyte cocultures in studies examining metal transport across the BBB.

Original languageEnglish (US)
Pages (from-to)235-243
Number of pages9
JournalJournal of Neuroscience Research
Issue number2
Publication statusPublished - Jul 15 2005



  • Astrocyte-conditioned medium
  • Energy dependence
  • Iron dependence
  • Proton dependence
  • Sodium dependence

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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