Manganese (Mn) transport across the rat blood-brain barrier: Saturable and transferrin-dependent transport mechanisms

Michael Aschner, Maureen Gannon

Research output: Contribution to journalArticle

161 Scopus citations

Abstract

Using a single capillary pass technique, the transport of manganese (Mn) across the rat blood-brain barrier (BBB) was characterized. Initial rate measurements (15 s) of Mn2+ [01000μM] accumulation in rat brains clearly indicated saturation kinetics by both 1/v vs. 1/s plots, and plots of v vs. [s]. Common carotid injection of freshly mixed Mn2+ with transferrin at a 1:10 molar ratio did not result in a significant change in the initial rate of Mn brain levels compared with injection of Mn2+ alone. However, when Mn2+ was incubated at 25°C in the presence of transferrin at a 1:10 ratio for up to 5 days prior to common carotid injection, the initial rate of Mn uptake by brain was incubationtime-dependent, increasing linearly with prolonged incubations. These findings suggest that the saturable component of divalent Mn transport into brain represents but one of the transport mechanisms for Mn across the BBB. A second transport system for Mn may occur by a transferrin-conjugated Mn transport system.

Original languageEnglish (US)
Pages (from-to)345-349
Number of pages5
JournalBrain Research Bulletin
Volume33
Issue number3
DOIs
StatePublished - 1994
Externally publishedYes

Keywords

  • Blood-Brain barrier
  • Manganese
  • Rat
  • Transferrin
  • Transport

ASJC Scopus subject areas

  • Neuroscience(all)

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