Mamo decodes hierarchical temporal gradients into terminal neuronal fate

Ling Yu Liu, Xi Long, Ching Po Yang, Rosa L. Miyares, Ken Sugino, Robert H. Singer, Tzumin Lee

Research output: Contribution to journalArticle

Abstract

Temporal patterning is a seminal method of expanding neuronal diversity. Here we unravel a mechanism decoding neural stem cell temporal gene expression and transforming it into discrete neuronal fates. This mechanism is characterized by hierarchical gene expression. First, Drosophila neuroblasts express opposing temporal gradients of RNA-binding proteins, Imp and Syp. These proteins promote or inhibit chinmo translation, yielding a descending neuronal gradient. Together, first and second-layer temporal factors define a temporal expression window of BTB-zinc finger nuclear protein, Mamo. The precise temporal induction of Mamo is achieved via both transcriptional and post-transcriptional regulation. Finally, Mamo is essential for the temporally defined, terminal identity of a’/b’ mushroom body neurons and identity maintenance. We describe a straightforward paradigm of temporal fate specification where diverse neuronal fates are defined via integrating multiple layers of gene regulation. The neurodevelopmental roles of orthologous/related mammalian genes suggest a fundamental conservation of this mechanism in brain development.

Original languageEnglish (US)
Article numbere48056
JournaleLife
Volume8
DOIs
StatePublished - Sep 2019

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Gene expression
RNA-Binding Proteins
Nuclear Proteins
Stem cells
Neurons
Decoding
Zinc
Conservation
Brain
Genes
Mushroom Bodies
Specifications
Gene Expression
Neural Stem Cells
Zinc Fingers
Drosophila
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Liu, L. Y., Long, X., Yang, C. P., Miyares, R. L., Sugino, K., Singer, R. H., & Lee, T. (2019). Mamo decodes hierarchical temporal gradients into terminal neuronal fate. eLife, 8, [e48056]. https://doi.org/10.7554/eLife.48056

Mamo decodes hierarchical temporal gradients into terminal neuronal fate. / Liu, Ling Yu; Long, Xi; Yang, Ching Po; Miyares, Rosa L.; Sugino, Ken; Singer, Robert H.; Lee, Tzumin.

In: eLife, Vol. 8, e48056, 09.2019.

Research output: Contribution to journalArticle

Liu, LY, Long, X, Yang, CP, Miyares, RL, Sugino, K, Singer, RH & Lee, T 2019, 'Mamo decodes hierarchical temporal gradients into terminal neuronal fate', eLife, vol. 8, e48056. https://doi.org/10.7554/eLife.48056
Liu, Ling Yu ; Long, Xi ; Yang, Ching Po ; Miyares, Rosa L. ; Sugino, Ken ; Singer, Robert H. ; Lee, Tzumin. / Mamo decodes hierarchical temporal gradients into terminal neuronal fate. In: eLife. 2019 ; Vol. 8.
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