Mammalian Maf1 Is a Negative Regulator of Transcription by All Three Nuclear RNA Polymerases

Sandra S. Johnson, Cheng Zhang, Jody Fromm, Ian M. Willis, Deborah L. Johnson

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Most eukaryotic transcriptional regulators act in an RNA polymerase (Pol)-selective manner. Here we show that the human Maf1 protein negatively regulates transcription by all three nuclear Pols. Changes in Maf1 expression affect Pol I- and Pol III-dependent transcription in human glioblastoma lines. These effects are mediated, in part, through the ability of Maf1 to repress transcription of the TATA binding protein, TBP. Maf1 targets an Elk-1-binding site in the TBP promoter, and its occupancy of this region is reciprocal with that of Elk-1. Similarly, Maf1 occupancy of Pol III genes is inversely correlated with that of the initiation factor TFIIIB and Pol III. The phenotypic consequences of reducing Maf1 expression include changes in cell morphology and the accumulation of actin stress fibers, whereas Maf1 overexpression suppresses anchorage-independent growth. Together with the ability of Maf1 to reduce biosynthetic capacity, these findings support the idea that Maf1 regulates the transformation state of cells.

Original languageEnglish (US)
Pages (from-to)367-379
Number of pages13
JournalMolecular Cell
Volume26
Issue number3
DOIs
StatePublished - May 11 2007

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Nuclear RNA
DNA-Directed RNA Polymerases
Transcription Factor TFIIIB
TATA-Box Binding Protein
Peptide Initiation Factors
Stress Fibers
Glioblastoma
Actins
Binding Sites
Growth
Genes
Proteins
tributyl phosphate

Keywords

  • DNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Mammalian Maf1 Is a Negative Regulator of Transcription by All Three Nuclear RNA Polymerases. / Johnson, Sandra S.; Zhang, Cheng; Fromm, Jody; Willis, Ian M.; Johnson, Deborah L.

In: Molecular Cell, Vol. 26, No. 3, 11.05.2007, p. 367-379.

Research output: Contribution to journalArticle

Johnson, Sandra S. ; Zhang, Cheng ; Fromm, Jody ; Willis, Ian M. ; Johnson, Deborah L. / Mammalian Maf1 Is a Negative Regulator of Transcription by All Three Nuclear RNA Polymerases. In: Molecular Cell. 2007 ; Vol. 26, No. 3. pp. 367-379.
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