Malaria infections do not compromise vaccine-induced immunity against tuberculosis in mice

Marcela Parra, Steven C. Derrick, Amy Yang, Jin Hua Tian, Kristopher Kolibab, Miranda Oakley, Liyanage P. Perera, William R. Jacobs, Sanjai Kumar, Sheldon L. Morris

Research output: Contribution to journalArticle

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Abstract

Background: Given the considerable geographic overlap in the endemic regions for malaria and tuberculosis, it is probable that co-infections with Mycobacterium tuberculosis and Plasmodium species are prevalent. Thus, it is quite likely that both malaria and TB vaccines may be used in the same populations in endemic areas. While novel vaccines are currently being developed and tested individually against each of these pathogens, the efficacy of these vaccines has not been evaluated in co-infection models. To further assess the effectiveness of these new immunization strategies, we investigated whether co-infection with malaria would impact the anti-tuberculosis protection induced by four different types of TB vaccines in a mouse model of pulmonary tuberculosis. Principal Findings: Here we show that the anti-tuberculosis protective immunity induced by four different tuberculosis vaccines was not impacted by a concurrent infection with Plasmodium yoelii NL, a nonlethal form of murine malaria. After an aerogenic challenge with virulent M. tuberculosis, the lung bacterial burdens of vaccinated animals were not statistically different in malaria infected and malaria naïve mice. Multi-parameter flow cytometric analysis showed that the frequency and the median fluorescence intensities (MFI) for specific multifunctional T (MFT) cells expressing IFN-γ, TNF-α, and/or IL-2 were suppressed by the presence of malaria parasites at 2 weeks following the malaria infection but was not affected after parasite clearance at 7 and 10 weeks post-challenge with P. yoelii NL. Conclusions: Our data indicate that the effectiveness of novel TB vaccines in protecting against tuberculosis was unaffected by a primary malaria co-infection in a mouse model of pulmonary tuberculosis. While the activities of specific MFT cell subsets were reduced at elevated levels of malaria parasitemia, the T cell suppression was short-lived. Our findings have important relevance in developing strategies for the deployment of new TB vaccines in malaria endemic areas.

Original languageEnglish (US)
Article numbere28164
JournalPloS one
Volume6
Issue number12
DOIs
StatePublished - Dec 19 2011

Fingerprint

tuberculosis
malaria
Malaria
Immunity
Tuberculosis
Vaccines
immunity
vaccines
T-cells
mice
Infection
mixed infection
infection
Coinfection
Plasmodium yoelii
Malaria Vaccines
Tuberculosis Vaccines
T-lymphocytes
lungs
Immunization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Parra, M., Derrick, S. C., Yang, A., Tian, J. H., Kolibab, K., Oakley, M., ... Morris, S. L. (2011). Malaria infections do not compromise vaccine-induced immunity against tuberculosis in mice. PloS one, 6(12), [e28164]. https://doi.org/10.1371/journal.pone.0028164

Malaria infections do not compromise vaccine-induced immunity against tuberculosis in mice. / Parra, Marcela; Derrick, Steven C.; Yang, Amy; Tian, Jin Hua; Kolibab, Kristopher; Oakley, Miranda; Perera, Liyanage P.; Jacobs, William R.; Kumar, Sanjai; Morris, Sheldon L.

In: PloS one, Vol. 6, No. 12, e28164, 19.12.2011.

Research output: Contribution to journalArticle

Parra, M, Derrick, SC, Yang, A, Tian, JH, Kolibab, K, Oakley, M, Perera, LP, Jacobs, WR, Kumar, S & Morris, SL 2011, 'Malaria infections do not compromise vaccine-induced immunity against tuberculosis in mice', PloS one, vol. 6, no. 12, e28164. https://doi.org/10.1371/journal.pone.0028164
Parra M, Derrick SC, Yang A, Tian JH, Kolibab K, Oakley M et al. Malaria infections do not compromise vaccine-induced immunity against tuberculosis in mice. PloS one. 2011 Dec 19;6(12). e28164. https://doi.org/10.1371/journal.pone.0028164
Parra, Marcela ; Derrick, Steven C. ; Yang, Amy ; Tian, Jin Hua ; Kolibab, Kristopher ; Oakley, Miranda ; Perera, Liyanage P. ; Jacobs, William R. ; Kumar, Sanjai ; Morris, Sheldon L. / Malaria infections do not compromise vaccine-induced immunity against tuberculosis in mice. In: PloS one. 2011 ; Vol. 6, No. 12.
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