Maintenance of mouse, rat, and pig pancreatic islet functions by coculture with human islet-derived fibroblasts

Atsushi Miki, Michiki Narushima, Teru Okitsu, Yuichi Takeno, Alejandro Soto-Gutierrez, Jorge David Rivas-Carrillo, Nalú Navarro-Alvarez, Yong Chen, Kimiaki Tanaka, Hirofumi Noguchi, Shinichi Matsumoto, Michinori Kohara, Jonathan R T Lakey, Eiji Kobayashi, Noriaki Tanaka, Naoya Kobayashi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Development of an efficient preculture system of islets is ideal. Toward that goal, we constructed a human pancreatic islet-derived fibroblast cell line MNNK-1 for a source as a coculture system for freshly isolated islets to maintain islet functions. Human pancreatic islet cells were nucleofected with a plasmid vector pYK-1 expressing simian virus 40 large T antigen gene (SV40T) and hygromycin resistance gene (HygroR). One of the transduced cell lines, MNNK-1, was established and served as a feeder cell in the coculture for freshly isolated mouse, rat, and pig islets. Morphology, viability, and glucose-responding insulin secretion were analyzed in the coculture system. MNNK-1 cells were morphologically spindle shaped and were negative for pancreatic endocrine markers. MNNK-1 cells were positive for α-smooth muscle actin and collagen type I and produced fibroblast growth factor. Coculture of the mouse, rat, and pig islets with MNNK-1 cells maintained their viability and insulin secretion with glucose responsiveness. A human pancreatic islet-derived fibroblast cell line MNNK-1 was established. MNNK-1 cells were a useful means for maintaining morphology and insulin secretion of islets in the coculture system.

Original languageEnglish (US)
Pages (from-to)325-334
Number of pages10
JournalCell Transplantation
Volume15
Issue number4
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Insulin
Fibroblasts
Coculture Techniques
Islets of Langerhans
Rats
Swine
Cells
Maintenance
Glucose
Genes
Cell Line
Antigens
Viruses
Collagen
Muscle
Feeder Cells
Simian virus 40
Fibroblast Growth Factors
Viral Tumor Antigens
Collagen Type I

Keywords

  • Coculture
  • Islets
  • Simian virus 40 large T antigen

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Miki, A., Narushima, M., Okitsu, T., Takeno, Y., Soto-Gutierrez, A., Rivas-Carrillo, J. D., ... Kobayashi, N. (2006). Maintenance of mouse, rat, and pig pancreatic islet functions by coculture with human islet-derived fibroblasts. Cell Transplantation, 15(4), 325-334. https://doi.org/10.3727/000000006783981882

Maintenance of mouse, rat, and pig pancreatic islet functions by coculture with human islet-derived fibroblasts. / Miki, Atsushi; Narushima, Michiki; Okitsu, Teru; Takeno, Yuichi; Soto-Gutierrez, Alejandro; Rivas-Carrillo, Jorge David; Navarro-Alvarez, Nalú; Chen, Yong; Tanaka, Kimiaki; Noguchi, Hirofumi; Matsumoto, Shinichi; Kohara, Michinori; Lakey, Jonathan R T; Kobayashi, Eiji; Tanaka, Noriaki; Kobayashi, Naoya.

In: Cell Transplantation, Vol. 15, No. 4, 2006, p. 325-334.

Research output: Contribution to journalArticle

Miki, A, Narushima, M, Okitsu, T, Takeno, Y, Soto-Gutierrez, A, Rivas-Carrillo, JD, Navarro-Alvarez, N, Chen, Y, Tanaka, K, Noguchi, H, Matsumoto, S, Kohara, M, Lakey, JRT, Kobayashi, E, Tanaka, N & Kobayashi, N 2006, 'Maintenance of mouse, rat, and pig pancreatic islet functions by coculture with human islet-derived fibroblasts', Cell Transplantation, vol. 15, no. 4, pp. 325-334. https://doi.org/10.3727/000000006783981882
Miki, Atsushi ; Narushima, Michiki ; Okitsu, Teru ; Takeno, Yuichi ; Soto-Gutierrez, Alejandro ; Rivas-Carrillo, Jorge David ; Navarro-Alvarez, Nalú ; Chen, Yong ; Tanaka, Kimiaki ; Noguchi, Hirofumi ; Matsumoto, Shinichi ; Kohara, Michinori ; Lakey, Jonathan R T ; Kobayashi, Eiji ; Tanaka, Noriaki ; Kobayashi, Naoya. / Maintenance of mouse, rat, and pig pancreatic islet functions by coculture with human islet-derived fibroblasts. In: Cell Transplantation. 2006 ; Vol. 15, No. 4. pp. 325-334.
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