Maea expressed by macrophages, but not erythroblasts, maintains postnatal murine bone marrow erythroblastic islands

Qiaozhi Wei, Philip E. Boulais, Dachuan Zhang, Sandra I. Pinho, Masato Tanaka, Paul S. Frenette

Research output: Contribution to journalArticle

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Abstract

The erythroblastic island (EI), formed by a central macrophage and developing erythroblasts (EBs), was first described decades ago and was recently shown to play an in vivo role in homeostatic and pathological erythropoiesis. The exact molecular mechanisms, however, mediating the interactions between macrophages and EBs remain unclear. Macrophage-EB attacher (Maea) has previously been suggested to mediate homophilic adhesion bounds bridging macrophages and EBs. Maea-deficient mice die perinatally with anemia and defective erythrocyte enucleation, suggesting a critical role in fetal erythropoiesis. Here, we generated conditional knockout mouse models of Maea to assess its cellular and postnatal contributions. Deletion of Maea in macrophages using Csf1r-Cre or CD169-Cre caused severe reductions of bone marrow (BM) macrophages, EBs, and in vivo island formation, whereas its deletion in the erythroid lineage using Epor-Cre had no such phenotype, suggesting a dominant role of Maea in the macrophage for BM erythropoiesis. Interestingly, Maea deletion in spleen macrophages did not alter their numbers or functions. Postnatal Maea deletion using Mx1-Cre or function inhibition using a novel monoclonal antibody also impaired BM erythropoiesis. These results indicate that Maea contributes to adult BM erythropoiesis by regulating the maintenance of macrophages and their interaction with EBs via an as-yet-unidentified EB receptor.

Original languageEnglish (US)
Pages (from-to)1222-1232
Number of pages11
JournalBlood
Volume133
Issue number11
DOIs
StatePublished - Mar 14 2019

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Erythroblasts
Macrophages
Bone
Bone Marrow
Erythropoiesis

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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Maea expressed by macrophages, but not erythroblasts, maintains postnatal murine bone marrow erythroblastic islands. / Wei, Qiaozhi; Boulais, Philip E.; Zhang, Dachuan; Pinho, Sandra I.; Tanaka, Masato; Frenette, Paul S.

In: Blood, Vol. 133, No. 11, 14.03.2019, p. 1222-1232.

Research output: Contribution to journalArticle

Wei, Qiaozhi ; Boulais, Philip E. ; Zhang, Dachuan ; Pinho, Sandra I. ; Tanaka, Masato ; Frenette, Paul S. / Maea expressed by macrophages, but not erythroblasts, maintains postnatal murine bone marrow erythroblastic islands. In: Blood. 2019 ; Vol. 133, No. 11. pp. 1222-1232.
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