Macrophage colony formation supported by purified CSF-1 and/or interleukin 3 in serum-free culture

Evidence for hierarchical difference in macrophage colony-forming cells

K. Koike, E. R. Stanley, J. N. Ihle, M. Ogawa

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Using a serum-free culture system, we examined murine macrophage colony formation from bone marrow cells cultured in the presence of purified CSF-1, interleukin 3 (IL 3) or a combination of the two factors. CSF-1 supported macrophage and neutrophil-macrophage colony formation, whereas IL-3 supported the formation of various types of single lineage and multilineage colonies. CSF-1 supported more macrophage colonies from bone marrow cells of normal mice than IL 3, whereas in cultures of bone marrow cells of 5-fluorouracil-treated mice, IL 3 supported more macrophage colonies. A combination of CSF-1 and IL 3 resulted in granulocyte-macrophage (GM) colony formation that was equal to or greater than the sum of GM colony formation supported by the factors individually. The combination of CSF-1 and IL 3 resulted in significant increases in the size of both macrophage and neutrophil-macrophage colonies. Similar increases in colony size were observed when CSF-1 was added to cultures five days after incubation of marrow cells with IL 3. These data support the concept that some of the macrophage colony-forming cells that respond to IL 3 are more primitive than those that are sensitive to CSF-1.

Original languageEnglish (US)
Pages (from-to)859-864
Number of pages6
JournalBlood
Volume67
Issue number4
StatePublished - 1986
Externally publishedYes

Fingerprint

Macrophage Colony-Stimulating Factor
Macrophages
Interleukin-3
Cell culture
Serum
Cells
Bone Marrow Cells
Bone
Granulocytes
Neutrophils
Fluorouracil
Bone Marrow

ASJC Scopus subject areas

  • Hematology

Cite this

Macrophage colony formation supported by purified CSF-1 and/or interleukin 3 in serum-free culture : Evidence for hierarchical difference in macrophage colony-forming cells. / Koike, K.; Stanley, E. R.; Ihle, J. N.; Ogawa, M.

In: Blood, Vol. 67, No. 4, 1986, p. 859-864.

Research output: Contribution to journalArticle

@article{9452073ac1ba4b04b938a83d1fdfdc8e,
title = "Macrophage colony formation supported by purified CSF-1 and/or interleukin 3 in serum-free culture: Evidence for hierarchical difference in macrophage colony-forming cells",
abstract = "Using a serum-free culture system, we examined murine macrophage colony formation from bone marrow cells cultured in the presence of purified CSF-1, interleukin 3 (IL 3) or a combination of the two factors. CSF-1 supported macrophage and neutrophil-macrophage colony formation, whereas IL-3 supported the formation of various types of single lineage and multilineage colonies. CSF-1 supported more macrophage colonies from bone marrow cells of normal mice than IL 3, whereas in cultures of bone marrow cells of 5-fluorouracil-treated mice, IL 3 supported more macrophage colonies. A combination of CSF-1 and IL 3 resulted in granulocyte-macrophage (GM) colony formation that was equal to or greater than the sum of GM colony formation supported by the factors individually. The combination of CSF-1 and IL 3 resulted in significant increases in the size of both macrophage and neutrophil-macrophage colonies. Similar increases in colony size were observed when CSF-1 was added to cultures five days after incubation of marrow cells with IL 3. These data support the concept that some of the macrophage colony-forming cells that respond to IL 3 are more primitive than those that are sensitive to CSF-1.",
author = "K. Koike and Stanley, {E. R.} and Ihle, {J. N.} and M. Ogawa",
year = "1986",
language = "English (US)",
volume = "67",
pages = "859--864",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "4",

}

TY - JOUR

T1 - Macrophage colony formation supported by purified CSF-1 and/or interleukin 3 in serum-free culture

T2 - Evidence for hierarchical difference in macrophage colony-forming cells

AU - Koike, K.

AU - Stanley, E. R.

AU - Ihle, J. N.

AU - Ogawa, M.

PY - 1986

Y1 - 1986

N2 - Using a serum-free culture system, we examined murine macrophage colony formation from bone marrow cells cultured in the presence of purified CSF-1, interleukin 3 (IL 3) or a combination of the two factors. CSF-1 supported macrophage and neutrophil-macrophage colony formation, whereas IL-3 supported the formation of various types of single lineage and multilineage colonies. CSF-1 supported more macrophage colonies from bone marrow cells of normal mice than IL 3, whereas in cultures of bone marrow cells of 5-fluorouracil-treated mice, IL 3 supported more macrophage colonies. A combination of CSF-1 and IL 3 resulted in granulocyte-macrophage (GM) colony formation that was equal to or greater than the sum of GM colony formation supported by the factors individually. The combination of CSF-1 and IL 3 resulted in significant increases in the size of both macrophage and neutrophil-macrophage colonies. Similar increases in colony size were observed when CSF-1 was added to cultures five days after incubation of marrow cells with IL 3. These data support the concept that some of the macrophage colony-forming cells that respond to IL 3 are more primitive than those that are sensitive to CSF-1.

AB - Using a serum-free culture system, we examined murine macrophage colony formation from bone marrow cells cultured in the presence of purified CSF-1, interleukin 3 (IL 3) or a combination of the two factors. CSF-1 supported macrophage and neutrophil-macrophage colony formation, whereas IL-3 supported the formation of various types of single lineage and multilineage colonies. CSF-1 supported more macrophage colonies from bone marrow cells of normal mice than IL 3, whereas in cultures of bone marrow cells of 5-fluorouracil-treated mice, IL 3 supported more macrophage colonies. A combination of CSF-1 and IL 3 resulted in granulocyte-macrophage (GM) colony formation that was equal to or greater than the sum of GM colony formation supported by the factors individually. The combination of CSF-1 and IL 3 resulted in significant increases in the size of both macrophage and neutrophil-macrophage colonies. Similar increases in colony size were observed when CSF-1 was added to cultures five days after incubation of marrow cells with IL 3. These data support the concept that some of the macrophage colony-forming cells that respond to IL 3 are more primitive than those that are sensitive to CSF-1.

UR - http://www.scopus.com/inward/record.url?scp=0022626623&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022626623&partnerID=8YFLogxK

M3 - Article

VL - 67

SP - 859

EP - 864

JO - Blood

JF - Blood

SN - 0006-4971

IS - 4

ER -