Macrophage autophagy limits acute toxic liver injury in mice through down regulation of interleukin-1β

Ghulam Ilyas, Enpeng Zhao, Kun Liu, Yu Lin, Lydia Tesfa, Kathryn E. Tanaka, Mark J. Czaja

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Background & Aims Overactivation of the innate immune response underlies many forms of liver injury including that caused by hepatotoxins. Recent studies have demonstrated that macrophage autophagy regulates innate immunity and resultant tissue inflammation. Although hepatocyte autophagy has been shown to modulate hepatic injury, little is known about the role of autophagy in hepatic macrophages during the inflammatory response to acute toxic liver injury. Our aim therefore was to determine whether macrophage autophagy functions to down regulate hepatic inflammation. Methods Mice with a LysM-CRE-mediated macrophage knockout of the autophagy gene ATG5 were examined for their response to toxin-induced liver injury from D-galactosamine/lipopolysaccharide (GalN/LPS). Results Knockout mice had increased liver injury from GalN/LPS as determined by significant increases in serum alanine aminotransferase, histological evidence of liver injury, positive terminal deoxynucleotide transferase-mediated deoxyuridine triphosphate nick end-labeling, caspase activation and mortality as compared to littermate controls. Levels of proinflammatory tumor necrosis factor and interleukin (IL)-6 hepatic mRNA and serum protein were unchanged, but serum IL-1β was significantly increased in knockout mice. The increase in serum IL-1β was secondary to elevated hepatic caspase 1 activation and inflammasome-mediated cleavage of pro-IL-1β to its active form. Cultured hepatic macrophages from GalN/LPS-treated knockout mice had similarly increased IL-1β production. Dysregulation of IL-1β was the mechanism of increased liver injury as an IL-1 receptor antagonist prevented injury in knockout mice in concert with decreased neutrophil activation. Conclusions Macrophage autophagy functions to limit acute toxin-induced liver injury and death by inhibiting the generation of inflammasome-dependent IL-1β.

Original languageEnglish (US)
Pages (from-to)118-127
Number of pages10
JournalJournal of Hepatology
Volume64
Issue number1
DOIs
StatePublished - 2016

Keywords

  • Apoptosis
  • Galactosamine
  • Inflammasome
  • Innate immunity
  • Kupffer cell
  • Lipopolysaccharide
  • Macrophage

ASJC Scopus subject areas

  • Hepatology

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