Lysophosphatidylcholine is a regulator of tyrosine kinase activity and intracellular Ca2+ level in Jurkat T cell line

Ádám Légrádi, Violeta Chitu, Valéria Szukacsov, Roberta Fajka-Boja, Kinga Székely Szücs, Éva Monostori

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Lysophospholipids, particularly lysophosphatidylcholine (lyso-PC), have been implicated in modulating T cell functions at the sites of inflammation and atherosclerosis. Although the chemotactic and immunomodulatory effects are well documented, the exact signaling pathway of lyso-PC action is poorly defined. In this work, we studied the earliest biochemical events in T cells triggered by lyso-PC. A marked and immediate tyrosine phosphorylation was induced in the leukemic T cell line, Jurkat. Phosphorylation of cellular substrates included src family kinase, p56lck and syk family kinase, ZAP70. The lyso-PC induced tyrosine phosphorylation was largely dependent on the presence of functional p56lck. Tyrosine phosphorylation was followed by the elevation of intracellular Ca2+ concentration. The magnitude of the mobilization of the intracellular Ca2+ was similar in the absence of the p56lck activity in JCaM1.6 cells as in Jurkat cells, however, it was slightly but reproducibly delayed compared to that in the wild type cells. Inhibition of the Ser/Thr kinases and tyrosine kinases with staurosporine and genistein, respectively, decreased the rise in the intracellular Ca 2+ content. Moreover, pertussis toxin completely blocked the Ca 2+ signal supporting the role of the G-protein coupled LPC receptor in this event.

Original languageEnglish (US)
Pages (from-to)17-21
Number of pages5
JournalImmunology Letters
Volume91
Issue number1
DOIs
StatePublished - Jan 30 2004

Keywords

  • Intracellular Ca
  • Lysophosphatidylcholine
  • Tyrosine phosphorylation
  • p56

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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