TY - JOUR
T1 - Lymphokine regulation of inflammatory processes
T2 - Interleukin-4 stimulates fibroblast proliferation
AU - Monroe, John G.
AU - Haldar, Subrata
AU - Prystowsky, Michael B.
AU - Lammie, Patrick
N1 - Funding Information:
We would like to thank Dr. John Cambier for providing us with D9Cl. 12.17 cells and Drs. Junichi Ohara and William Paul for the llBl1 hybridoma. This work was supported by funds from the NIH. W. W. Smith Charitable Trust, Hartford Foundation, and the American Cancer Society.
PY - 1988/11
Y1 - 1988/11
N2 - While recent evidence from several laboratories has shown that interleukin-4 (IL-4) can act on a number of cells in addition to B lymphocytes, these have thus far been limited to cells of the hematopoietic lineage. Here we report that murine IL-4 promotes DNA synthesis in both primary and immortalized fibroblasts. Marked stimulation of [3H]thymidine incorporation was observed for primary skin fibroblasts of Balb/c3T3 cells stimulated with HPLC- or immunoaffinity-purified as well as recombinant IL-4. Responses to immunoaffinity and recombinant IL-4 were completely blocked with anti-IL-4 antibody. Similar dose/response relationships were observed for recombinant IL-4 on skin fibroblasts and an IL-4 responsive murine T cell tumor, suggesting that the receptors for this lymphokine on these cells is simular. Together, these results show that IL-4 can cause DNA synthesis by murine fibroblasts presumably through ligand-receptor interactions at the cell surface. Implications of these findings to inflammation during an immune response is discussed.
AB - While recent evidence from several laboratories has shown that interleukin-4 (IL-4) can act on a number of cells in addition to B lymphocytes, these have thus far been limited to cells of the hematopoietic lineage. Here we report that murine IL-4 promotes DNA synthesis in both primary and immortalized fibroblasts. Marked stimulation of [3H]thymidine incorporation was observed for primary skin fibroblasts of Balb/c3T3 cells stimulated with HPLC- or immunoaffinity-purified as well as recombinant IL-4. Responses to immunoaffinity and recombinant IL-4 were completely blocked with anti-IL-4 antibody. Similar dose/response relationships were observed for recombinant IL-4 on skin fibroblasts and an IL-4 responsive murine T cell tumor, suggesting that the receptors for this lymphokine on these cells is simular. Together, these results show that IL-4 can cause DNA synthesis by murine fibroblasts presumably through ligand-receptor interactions at the cell surface. Implications of these findings to inflammation during an immune response is discussed.
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U2 - 10.1016/0090-1229(88)90119-5
DO - 10.1016/0090-1229(88)90119-5
M3 - Article
C2 - 3262472
AN - SCOPUS:0023698689
SN - 0090-1229
VL - 49
SP - 292
EP - 298
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
IS - 2
ER -