Lymphokine regulation of inflammatory processes: Interleukin-4 stimulates fibroblast proliferation

John G. Monroe, Subrata Haldar, Michael B. Prystowsky, Patrick Lammie

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

While recent evidence from several laboratories has shown that interleukin-4 (IL-4) can act on a number of cells in addition to B lymphocytes, these have thus far been limited to cells of the hematopoietic lineage. Here we report that murine IL-4 promotes DNA synthesis in both primary and immortalized fibroblasts. Marked stimulation of [3H]thymidine incorporation was observed for primary skin fibroblasts of Balb/c3T3 cells stimulated with HPLC- or immunoaffinity-purified as well as recombinant IL-4. Responses to immunoaffinity and recombinant IL-4 were completely blocked with anti-IL-4 antibody. Similar dose/response relationships were observed for recombinant IL-4 on skin fibroblasts and an IL-4 responsive murine T cell tumor, suggesting that the receptors for this lymphokine on these cells is simular. Together, these results show that IL-4 can cause DNA synthesis by murine fibroblasts presumably through ligand-receptor interactions at the cell surface. Implications of these findings to inflammation during an immune response is discussed.

Original languageEnglish (US)
Pages (from-to)292-298
Number of pages7
JournalClinical Immunology and Immunopathology
Volume49
Issue number2
DOIs
Publication statusPublished - Nov 1988
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

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