Lymphokine regulation of inflammatory processes

Interleukin-4 stimulates fibroblast proliferation

John G. Monroe, Subrata Haldar, Michael B. Prystowsky, Patrick Lammie

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

While recent evidence from several laboratories has shown that interleukin-4 (IL-4) can act on a number of cells in addition to B lymphocytes, these have thus far been limited to cells of the hematopoietic lineage. Here we report that murine IL-4 promotes DNA synthesis in both primary and immortalized fibroblasts. Marked stimulation of [3H]thymidine incorporation was observed for primary skin fibroblasts of Balb/c3T3 cells stimulated with HPLC- or immunoaffinity-purified as well as recombinant IL-4. Responses to immunoaffinity and recombinant IL-4 were completely blocked with anti-IL-4 antibody. Similar dose/response relationships were observed for recombinant IL-4 on skin fibroblasts and an IL-4 responsive murine T cell tumor, suggesting that the receptors for this lymphokine on these cells is simular. Together, these results show that IL-4 can cause DNA synthesis by murine fibroblasts presumably through ligand-receptor interactions at the cell surface. Implications of these findings to inflammation during an immune response is discussed.

Original languageEnglish (US)
Pages (from-to)292-298
Number of pages7
JournalClinical Immunology and Immunopathology
Volume49
Issue number2
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Lymphokines
Interleukin-4
Fibroblasts
Skin
DNA
Cell Lineage
Cell Communication
Thymidine
B-Lymphocytes
Cell Count
High Pressure Liquid Chromatography
Ligands
Inflammation
T-Lymphocytes
Antibodies

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Lymphokine regulation of inflammatory processes : Interleukin-4 stimulates fibroblast proliferation. / Monroe, John G.; Haldar, Subrata; Prystowsky, Michael B.; Lammie, Patrick.

In: Clinical Immunology and Immunopathology, Vol. 49, No. 2, 1988, p. 292-298.

Research output: Contribution to journalArticle

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