Lymphoid lineage-associated features in acute myeloid leukaemia: Phenotypic and genotypic correlations

Elisabeth M. Paietta, B. Van Ness, J. Bennett, Janis Racevskis, Rasim A. Gucalp, P. Cassileth, P. H. Wiernik

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

This study is intended to establish biological correlation between the expression of lymphoid associated features in acute myeloid leukaemia (AML). In 62 AML patients, predominantly enrolled on Eastern Cooperative Oncology Group (ECOG) treatment protocols, in whom immunoglobulin (Ig) as well as T-cell receptor beta chain (TCR-beta) gene rearrangement analyses had been performed, morphology, cytochemistry, antigen profile and karyotype were reviewed retrospectively. Nuclear reactivity with anti-TdT antibody was demonstrated in 34 patients (55%) and confirmed by ribonuclease protection assay in all patients tested. Five TdT-protein negative patients were TdT-transcript positive. Lymphoid antigens (lyA) were detected in 24 of 51 cases tested (47%) with B-cell antigens (CD19, CD10) being restricted to TdT+ AML (P = 0.03). Only two patients had Ig heavy, none had Ig light chain or TCR-beta gene rearrangements. Although both patients with rearranged Ig loci were TdT+, either by protein or RNA analysis, the low incidence of such rearrangement within the TdT+ AML group (6%) argues against a significant association between the presence of TdT and crosslineage Ig gene rearrangements in AML. While FAB-diagnoses did not differ between TdT+ and TdT- or lyA+ and lyA- AML, particular immunophenotypic features correlated with TdT positively, e.g. the presence of early antigens, CD34 and HLA-DR, and the absence of the more mature myelo-monocytic antigens, CDw65 and CD14. Certain cytogenetic abnormalities were associated with TdT+ AML such as inv(16) (p13q22) or t(16;16) (p12;q22) (five patients; P = 0.03) and t(8;21) (q22;q22) (three patients). A greater number of TdT- than TdT+ AML patients had only normal karyotypes (P = 0.06). Neither immunophenotypic nor karyotypic correlations could be established for lyA+ AML.

Original languageEnglish (US)
Pages (from-to)324-331
Number of pages8
JournalBritish Journal of Haematology
Volume82
Issue number2
StatePublished - 1992

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Acute Myeloid Leukemia
beta-Chain T-Cell Antigen Receptor Gene Rearrangement
Antigens
T-Cell Receptor beta Genes
Immunoglobulins
Karyotype
CD19 Antigens
CD14 Antigens
Immunoglobulin Light Chains
Histocytochemistry
Immunoglobulin Genes
Gene Rearrangement
HLA-DR Antigens
Ribonucleases
Clinical Protocols
Chromosome Aberrations
Anti-Idiotypic Antibodies
Proteins
RNA
Incidence

ASJC Scopus subject areas

  • Hematology

Cite this

Lymphoid lineage-associated features in acute myeloid leukaemia : Phenotypic and genotypic correlations. / Paietta, Elisabeth M.; Van Ness, B.; Bennett, J.; Racevskis, Janis; Gucalp, Rasim A.; Cassileth, P.; Wiernik, P. H.

In: British Journal of Haematology, Vol. 82, No. 2, 1992, p. 324-331.

Research output: Contribution to journalArticle

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abstract = "This study is intended to establish biological correlation between the expression of lymphoid associated features in acute myeloid leukaemia (AML). In 62 AML patients, predominantly enrolled on Eastern Cooperative Oncology Group (ECOG) treatment protocols, in whom immunoglobulin (Ig) as well as T-cell receptor beta chain (TCR-beta) gene rearrangement analyses had been performed, morphology, cytochemistry, antigen profile and karyotype were reviewed retrospectively. Nuclear reactivity with anti-TdT antibody was demonstrated in 34 patients (55{\%}) and confirmed by ribonuclease protection assay in all patients tested. Five TdT-protein negative patients were TdT-transcript positive. Lymphoid antigens (lyA) were detected in 24 of 51 cases tested (47{\%}) with B-cell antigens (CD19, CD10) being restricted to TdT+ AML (P = 0.03). Only two patients had Ig heavy, none had Ig light chain or TCR-beta gene rearrangements. Although both patients with rearranged Ig loci were TdT+, either by protein or RNA analysis, the low incidence of such rearrangement within the TdT+ AML group (6{\%}) argues against a significant association between the presence of TdT and crosslineage Ig gene rearrangements in AML. While FAB-diagnoses did not differ between TdT+ and TdT- or lyA+ and lyA- AML, particular immunophenotypic features correlated with TdT positively, e.g. the presence of early antigens, CD34 and HLA-DR, and the absence of the more mature myelo-monocytic antigens, CDw65 and CD14. Certain cytogenetic abnormalities were associated with TdT+ AML such as inv(16) (p13q22) or t(16;16) (p12;q22) (five patients; P = 0.03) and t(8;21) (q22;q22) (three patients). A greater number of TdT- than TdT+ AML patients had only normal karyotypes (P = 0.06). Neither immunophenotypic nor karyotypic correlations could be established for lyA+ AML.",
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