TY - JOUR
T1 - Lymphocyte-specific inducible expression of potassium channel beta subunits
AU - Autieri, Michael V.
AU - Belkowski, Stanley M.
AU - Constantinescu, Cristian S.
AU - Cohen, Jeffrey A.
AU - Prystowsky, Michael B.
N1 - Funding Information:
M.B.P. is the recipient of an American Cancer Society Faculty Research Award. Supported in part by grant MH51327 from the NIMH and Yeshiva University BRSG No. 9-526-0522 to M.B.P.
PY - 1997/7
Y1 - 1997/7
N2 - Many studies have shown that voltage-gated potassium (Kv) channel activity is essential for T-lymphocyte proliferation. The IL-2-inducible neuroimmune gene, 12rf5 is the mouse homologue of the rat Kvβ2 subunit. In this study we show that in addition to constitutive expression in adult murine brain, expression of Kv channel subunits β 1.1 and β 2.1 is inducible in a cloned T-helper cell line stimulated with IL-2 and in normal murine splenocytes stimulated with Con A or LPS. This expression pattern appears to be lymphocyte specific, because stimulated fibroblasts and vascular smooth muscle cells do not express Kvβ channel subunit mRNA. These observations suggest that Kvβ subunit expression is tissue specific and inducible in stimulated lymphocytes. Because Kvβ subunits modulate K+ channel activity, the inducible and variable expression of these subunits in lymphocytes may represent an additional regulatory mechanism for lymphocyte proliferation.
AB - Many studies have shown that voltage-gated potassium (Kv) channel activity is essential for T-lymphocyte proliferation. The IL-2-inducible neuroimmune gene, 12rf5 is the mouse homologue of the rat Kvβ2 subunit. In this study we show that in addition to constitutive expression in adult murine brain, expression of Kv channel subunits β 1.1 and β 2.1 is inducible in a cloned T-helper cell line stimulated with IL-2 and in normal murine splenocytes stimulated with Con A or LPS. This expression pattern appears to be lymphocyte specific, because stimulated fibroblasts and vascular smooth muscle cells do not express Kvβ channel subunit mRNA. These observations suggest that Kvβ subunit expression is tissue specific and inducible in stimulated lymphocytes. Because Kvβ subunits modulate K+ channel activity, the inducible and variable expression of these subunits in lymphocytes may represent an additional regulatory mechanism for lymphocyte proliferation.
KW - B subunits
KW - IL-2
KW - Lymphocyte activation
KW - Potassium channels
KW - T-cells
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U2 - 10.1016/S0165-5728(97)00050-7
DO - 10.1016/S0165-5728(97)00050-7
M3 - Article
C2 - 9209263
AN - SCOPUS:0030852099
SN - 0165-5728
VL - 77
SP - 8
EP - 16
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1
ER -