Lymphocyte changes in favism: In vitro evidence of a modifying effect of bilirubin and hemoglobin on t-lymphocyte receptors

Gino Schilirò, Antonella Sciotto, Antonio Russo, Gaetano Bottaro, Caterina P. Minniti, Salvatore Musumeci, Giuseppe Russo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Lymphocyte subpopulations were studied in 13 Sicilian glucose-6-phosphate dehydrogenase (G6PD)-deficient children during and after the hemolytic crisis due to fava bean ingestion. A statistically significant reduction of sheep-red-cell (SRC)-rosetting lymphocytes occurred in all patients during the hemolytic crisis, whereas B lymphocytes were not affected. In order to establish the possible relationship between serum changes occurring during hemolysis and reduction of SRC-rosetting lymphocytes, the effect in vitro of varying concentrations of bilirubin and hemoglobin on the rosette formation capacity of lymphocytes was studied. Both substances produced a statistically significant reduction of SRC-rosetting lymphocytes both in normal and deficient subjects. This effect showed a direct relationship with the concentrations of bilirubin and hemoglobin used and was more pronounced on lymphocytes from G6PD-deficient children. These data suggested that the reduction of SRC-rosetting lymphocytes observed during acute hemolysis of favism can be related to a modifying effect of bilirubin and hemoglobin.

Original languageEnglish (US)
Pages (from-to)230-235
Number of pages6
JournalActa Haematologica
Volume69
Issue number4
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Favism
Bilirubin
Hemoglobins
Lymphocytes
Sheep
Glucosephosphate Dehydrogenase
Hemolysis
Rosette Formation
Vicia faba
Lymphocyte Subsets
In Vitro Techniques
B-Lymphocytes
Eating
Serum

Keywords

  • Favism
  • G6PD deficiency
  • SRC-rosetting lymphocytes

ASJC Scopus subject areas

  • Medicine(all)
  • Hematology

Cite this

Lymphocyte changes in favism : In vitro evidence of a modifying effect of bilirubin and hemoglobin on t-lymphocyte receptors. / Schilirò, Gino; Sciotto, Antonella; Russo, Antonio; Bottaro, Gaetano; Minniti, Caterina P.; Musumeci, Salvatore; Russo, Giuseppe.

In: Acta Haematologica, Vol. 69, No. 4, 1983, p. 230-235.

Research output: Contribution to journalArticle

Schilirò, Gino ; Sciotto, Antonella ; Russo, Antonio ; Bottaro, Gaetano ; Minniti, Caterina P. ; Musumeci, Salvatore ; Russo, Giuseppe. / Lymphocyte changes in favism : In vitro evidence of a modifying effect of bilirubin and hemoglobin on t-lymphocyte receptors. In: Acta Haematologica. 1983 ; Vol. 69, No. 4. pp. 230-235.
@article{8e7581aeb826453ab4cafb4860cd69ff,
title = "Lymphocyte changes in favism: In vitro evidence of a modifying effect of bilirubin and hemoglobin on t-lymphocyte receptors",
abstract = "Lymphocyte subpopulations were studied in 13 Sicilian glucose-6-phosphate dehydrogenase (G6PD)-deficient children during and after the hemolytic crisis due to fava bean ingestion. A statistically significant reduction of sheep-red-cell (SRC)-rosetting lymphocytes occurred in all patients during the hemolytic crisis, whereas B lymphocytes were not affected. In order to establish the possible relationship between serum changes occurring during hemolysis and reduction of SRC-rosetting lymphocytes, the effect in vitro of varying concentrations of bilirubin and hemoglobin on the rosette formation capacity of lymphocytes was studied. Both substances produced a statistically significant reduction of SRC-rosetting lymphocytes both in normal and deficient subjects. This effect showed a direct relationship with the concentrations of bilirubin and hemoglobin used and was more pronounced on lymphocytes from G6PD-deficient children. These data suggested that the reduction of SRC-rosetting lymphocytes observed during acute hemolysis of favism can be related to a modifying effect of bilirubin and hemoglobin.",
keywords = "Favism, G6PD deficiency, SRC-rosetting lymphocytes",
author = "Gino Schilir{\`o} and Antonella Sciotto and Antonio Russo and Gaetano Bottaro and Minniti, {Caterina P.} and Salvatore Musumeci and Giuseppe Russo",
year = "1983",
doi = "10.1159/000206897",
language = "English (US)",
volume = "69",
pages = "230--235",
journal = "Acta Haematologica",
issn = "0001-5792",
publisher = "S. Karger AG",
number = "4",

}

TY - JOUR

T1 - Lymphocyte changes in favism

T2 - In vitro evidence of a modifying effect of bilirubin and hemoglobin on t-lymphocyte receptors

AU - Schilirò, Gino

AU - Sciotto, Antonella

AU - Russo, Antonio

AU - Bottaro, Gaetano

AU - Minniti, Caterina P.

AU - Musumeci, Salvatore

AU - Russo, Giuseppe

PY - 1983

Y1 - 1983

N2 - Lymphocyte subpopulations were studied in 13 Sicilian glucose-6-phosphate dehydrogenase (G6PD)-deficient children during and after the hemolytic crisis due to fava bean ingestion. A statistically significant reduction of sheep-red-cell (SRC)-rosetting lymphocytes occurred in all patients during the hemolytic crisis, whereas B lymphocytes were not affected. In order to establish the possible relationship between serum changes occurring during hemolysis and reduction of SRC-rosetting lymphocytes, the effect in vitro of varying concentrations of bilirubin and hemoglobin on the rosette formation capacity of lymphocytes was studied. Both substances produced a statistically significant reduction of SRC-rosetting lymphocytes both in normal and deficient subjects. This effect showed a direct relationship with the concentrations of bilirubin and hemoglobin used and was more pronounced on lymphocytes from G6PD-deficient children. These data suggested that the reduction of SRC-rosetting lymphocytes observed during acute hemolysis of favism can be related to a modifying effect of bilirubin and hemoglobin.

AB - Lymphocyte subpopulations were studied in 13 Sicilian glucose-6-phosphate dehydrogenase (G6PD)-deficient children during and after the hemolytic crisis due to fava bean ingestion. A statistically significant reduction of sheep-red-cell (SRC)-rosetting lymphocytes occurred in all patients during the hemolytic crisis, whereas B lymphocytes were not affected. In order to establish the possible relationship between serum changes occurring during hemolysis and reduction of SRC-rosetting lymphocytes, the effect in vitro of varying concentrations of bilirubin and hemoglobin on the rosette formation capacity of lymphocytes was studied. Both substances produced a statistically significant reduction of SRC-rosetting lymphocytes both in normal and deficient subjects. This effect showed a direct relationship with the concentrations of bilirubin and hemoglobin used and was more pronounced on lymphocytes from G6PD-deficient children. These data suggested that the reduction of SRC-rosetting lymphocytes observed during acute hemolysis of favism can be related to a modifying effect of bilirubin and hemoglobin.

KW - Favism

KW - G6PD deficiency

KW - SRC-rosetting lymphocytes

UR - http://www.scopus.com/inward/record.url?scp=0020686580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020686580&partnerID=8YFLogxK

U2 - 10.1159/000206897

DO - 10.1159/000206897

M3 - Article

C2 - 6402878

AN - SCOPUS:0020686580

VL - 69

SP - 230

EP - 235

JO - Acta Haematologica

JF - Acta Haematologica

SN - 0001-5792

IS - 4

ER -