Lupus anticoagulant increases activated partial thromboplastin time (PTT) prolongation in incubated 1: 1 mix.

Grace Monis, Chris Ferrell, Morayma Reyes Gil

Research output: Contribution to journalArticle

Abstract

Our study analyzes the effects of incubation time and strength of lupus anticoagulant (LAC) on clotting times and prolongation of activated partial thromboplastin time (PTT) 1:1 mix assays with incubation. The prolongation in seconds of PTT 1:1 mix after incubation in the confirmed presence or absence of LAC was correlated to strength of the LAC as well as length of incubation (1 vs. 2 hours). Our study suggests that when screening for possible Factor VIII (FVIII) inhibitors, a 2 hour incubation of a PTT 1:1 mix increases the frequency of false positives as compared to 1 hour incubation, and that most of these false positives are due to LACs. Prolongation of clotting times for PTT 1:1 mixes in patients with LAC is influenced by both length of incubation time and strength of the LAC. Conclusions: When using PTT 1:1 mixes to screen for FVIII inhibitors, the effect of a possible LAC on the interpretation of the PTT prolongation should be considered. This effect is influenced by both incubation time and LAC strength.

Original languageEnglish (US)
Pages (from-to)165-169
Number of pages5
JournalClinical Laboratory Science
Volume25
Issue number3
StatePublished - Jun 2012
Externally publishedYes

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Lupus Coagulation Inhibitor
Partial Thromboplastin Time
Thromboplastin
Factor VIII
Assays
Screening

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lupus anticoagulant increases activated partial thromboplastin time (PTT) prolongation in incubated 1 : 1 mix. / Monis, Grace; Ferrell, Chris; Reyes Gil, Morayma.

In: Clinical Laboratory Science, Vol. 25, No. 3, 06.2012, p. 165-169.

Research output: Contribution to journalArticle

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