Lumen morphogenesis in C. elegans requires the membrane-cytoskeleton linker erm-1

Verena Göbel, Peter L. Barrett, David H. Hall, John T. Fleming

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Epithelial tubes are basic building blocks of complex organs, but their architectural requirements are not well understood. Here we show that erm-1 is a unique C. elegans ortholog of the ERM family of cytoskeleton-membrane linkers, with an essential role in lumen morphogenesis. ERM-1 localizes to the luminal membranes of those tubular organ epithelia which lack stabilization by cuticle. RNA interference (RNAi), a germline deletion, and overexpression of erm-1 cause cystic luminal phenotypes in these epithelia. Confocal and ultrastructural analyses indicate that erm-1 functions directly in apical membrane morphogenesis, rather than in epithelial polarity and junction assembly as has been previously proposed for ERMs. We also show that act-5/cytoplasmic actin and sma-1/β-H-spectrin are required for lumen formation and functionally interact with erm-1. Our findings suggest that there are common structural constraints on the architecture of diverse organ lumina.

Original languageEnglish (US)
Pages (from-to)865-873
Number of pages9
JournalDevelopmental Cell
Volume6
Issue number6
DOIs
StatePublished - Jun 2004

Fingerprint

Cytoskeleton
Morphogenesis
Membranes
Epithelium
Spectrin
RNA Interference
Actins
Stabilization
RNA
Phenotype

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Lumen morphogenesis in C. elegans requires the membrane-cytoskeleton linker erm-1. / Göbel, Verena; Barrett, Peter L.; Hall, David H.; Fleming, John T.

In: Developmental Cell, Vol. 6, No. 6, 06.2004, p. 865-873.

Research output: Contribution to journalArticle

Göbel, Verena ; Barrett, Peter L. ; Hall, David H. ; Fleming, John T. / Lumen morphogenesis in C. elegans requires the membrane-cytoskeleton linker erm-1. In: Developmental Cell. 2004 ; Vol. 6, No. 6. pp. 865-873.
@article{87fd91cf00e6458ea8ecb1f59ac4d11a,
title = "Lumen morphogenesis in C. elegans requires the membrane-cytoskeleton linker erm-1",
abstract = "Epithelial tubes are basic building blocks of complex organs, but their architectural requirements are not well understood. Here we show that erm-1 is a unique C. elegans ortholog of the ERM family of cytoskeleton-membrane linkers, with an essential role in lumen morphogenesis. ERM-1 localizes to the luminal membranes of those tubular organ epithelia which lack stabilization by cuticle. RNA interference (RNAi), a germline deletion, and overexpression of erm-1 cause cystic luminal phenotypes in these epithelia. Confocal and ultrastructural analyses indicate that erm-1 functions directly in apical membrane morphogenesis, rather than in epithelial polarity and junction assembly as has been previously proposed for ERMs. We also show that act-5/cytoplasmic actin and sma-1/β-H-spectrin are required for lumen formation and functionally interact with erm-1. Our findings suggest that there are common structural constraints on the architecture of diverse organ lumina.",
author = "Verena G{\"o}bel and Barrett, {Peter L.} and Hall, {David H.} and Fleming, {John T.}",
year = "2004",
month = "6",
doi = "10.1016/j.devcel.2004.05.018",
language = "English (US)",
volume = "6",
pages = "865--873",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "6",

}

TY - JOUR

T1 - Lumen morphogenesis in C. elegans requires the membrane-cytoskeleton linker erm-1

AU - Göbel, Verena

AU - Barrett, Peter L.

AU - Hall, David H.

AU - Fleming, John T.

PY - 2004/6

Y1 - 2004/6

N2 - Epithelial tubes are basic building blocks of complex organs, but their architectural requirements are not well understood. Here we show that erm-1 is a unique C. elegans ortholog of the ERM family of cytoskeleton-membrane linkers, with an essential role in lumen morphogenesis. ERM-1 localizes to the luminal membranes of those tubular organ epithelia which lack stabilization by cuticle. RNA interference (RNAi), a germline deletion, and overexpression of erm-1 cause cystic luminal phenotypes in these epithelia. Confocal and ultrastructural analyses indicate that erm-1 functions directly in apical membrane morphogenesis, rather than in epithelial polarity and junction assembly as has been previously proposed for ERMs. We also show that act-5/cytoplasmic actin and sma-1/β-H-spectrin are required for lumen formation and functionally interact with erm-1. Our findings suggest that there are common structural constraints on the architecture of diverse organ lumina.

AB - Epithelial tubes are basic building blocks of complex organs, but their architectural requirements are not well understood. Here we show that erm-1 is a unique C. elegans ortholog of the ERM family of cytoskeleton-membrane linkers, with an essential role in lumen morphogenesis. ERM-1 localizes to the luminal membranes of those tubular organ epithelia which lack stabilization by cuticle. RNA interference (RNAi), a germline deletion, and overexpression of erm-1 cause cystic luminal phenotypes in these epithelia. Confocal and ultrastructural analyses indicate that erm-1 functions directly in apical membrane morphogenesis, rather than in epithelial polarity and junction assembly as has been previously proposed for ERMs. We also show that act-5/cytoplasmic actin and sma-1/β-H-spectrin are required for lumen formation and functionally interact with erm-1. Our findings suggest that there are common structural constraints on the architecture of diverse organ lumina.

UR - http://www.scopus.com/inward/record.url?scp=2942626080&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942626080&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2004.05.018

DO - 10.1016/j.devcel.2004.05.018

M3 - Article

VL - 6

SP - 865

EP - 873

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 6

ER -