Lumen morphogenesis in C. elegans requires the membrane-cytoskeleton linker erm-1

Verena Göbel, Peter L. Barrett, David H. Hall, John T. Fleming

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

Epithelial tubes are basic building blocks of complex organs, but their architectural requirements are not well understood. Here we show that erm-1 is a unique C. elegans ortholog of the ERM family of cytoskeleton-membrane linkers, with an essential role in lumen morphogenesis. ERM-1 localizes to the luminal membranes of those tubular organ epithelia which lack stabilization by cuticle. RNA interference (RNAi), a germline deletion, and overexpression of erm-1 cause cystic luminal phenotypes in these epithelia. Confocal and ultrastructural analyses indicate that erm-1 functions directly in apical membrane morphogenesis, rather than in epithelial polarity and junction assembly as has been previously proposed for ERMs. We also show that act-5/cytoplasmic actin and sma-1/β-H-spectrin are required for lumen formation and functionally interact with erm-1. Our findings suggest that there are common structural constraints on the architecture of diverse organ lumina.

Original languageEnglish (US)
Pages (from-to)865-873
Number of pages9
JournalDevelopmental cell
Volume6
Issue number6
DOIs
StatePublished - Jun 1 2004

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Lumen morphogenesis in C. elegans requires the membrane-cytoskeleton linker erm-1'. Together they form a unique fingerprint.

  • Cite this