LspA-independent action of globomycin on Mycobacterium tuberculosis

Niaz Banaiee, William R. Jacobs, Joel D. Ernst

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Objectives: The objective of this study was to investigate the antimicrobial activity and specificity of globomycin, an inhibitor of lipoprotein signal peptidase II (LspA), against Mycobacterium tuberculosis. Methods: The mycobactericidal and mycobacteriostatic activity of globomycin was determined by optical density and cfu plating. The specificity of globomycin was determined by western immunoblotting using anti-MPT83 antibody. Results: Globomycin is mycobactericidal at concentrations ≥40 mg/L. However, at 80 mg/L, the processing of the lipoprotein MPT-83 is unaffected and growth-inhibitory effect of globomycin is unchanged in an lspA null mutant (Δ lspA:: lspAmut) lacking the putative drug target. Conclusions: Globomycin kills M. tuberculosis through a mechanism that is independent of LspA.

Original languageEnglish (US)
Pages (from-to)414-416
Number of pages3
JournalJournal of Antimicrobial Chemotherapy
Volume60
Issue number2
DOIs
StatePublished - Aug 1 2007

Keywords

  • Antimycobacterials
  • Lipoprotein processing
  • Lipoprotein signal peptidase
  • Lipoproteins

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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