Low-M(r) iron isolated from guinea pig reticulocytes as AMP-Fe and ATP-Fe complexes

J. Weaver, S. Pollack

Research output: Contribution to journalArticle

112 Scopus citations

Abstract

Guinea pig reticulocytes were pulse-labelled with 59Fe bound to transferrin. Haemolysates prepared from these reticulocytes were subjected to rapid (NH4)2SO4 precipitation and then chromatography on an anion-exchange resin. ATP-bound 59Fe was the dominant species in the reticulocyte cytosol; 2,3-bisphosphoglycerate and GTP iron complexes were not detected despite the fact that these were stable with (NH4)2SO4 precipitation and readily detected with anion-exchange chromatography. AMP-bound Fe was a minor component of the cytosol following rapid (NH4)2SO4 precipitation, and the major component when iron was released from transferrin by haemolysates. We speculate that ATP-Fe may be degraded in the cell to permit utilization of its iron for haem synthesis.

Original languageEnglish (US)
Pages (from-to)787-792
Number of pages6
JournalBiochemical Journal
Volume261
Issue number3
DOIs
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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