TY - JOUR
T1 - Low molecular weight nonheme iron and a highly labeled heme pool in the reticulocyte
AU - Pollack, S.
AU - Campana, T.
PY - 1980
Y1 - 1980
N2 - The ligands that bind iron and heme in the reticulocyte and may regulate their distribution are not known. Guinea pig reticulocyte hemolysates were labeled with 59Fe and filtered through molecular weight sieving columns. 59Fe-containing peaks of ferritin, transferrin, and hemoglobin were identified, as well as a low molecular weight peak containing predominantly nonheme iron, and a 17,000 mol wt heme containing peak with a specific activity (59Fe/heme) 20-fold greater than hemoglobin. We note that the low molecular weight peak, the existence of which has been postulated, has been identified for the first time without the addition of a chelating agent. We speculate that the 17,000 mol wt peak is an α-chain pool.
AB - The ligands that bind iron and heme in the reticulocyte and may regulate their distribution are not known. Guinea pig reticulocyte hemolysates were labeled with 59Fe and filtered through molecular weight sieving columns. 59Fe-containing peaks of ferritin, transferrin, and hemoglobin were identified, as well as a low molecular weight peak containing predominantly nonheme iron, and a 17,000 mol wt heme containing peak with a specific activity (59Fe/heme) 20-fold greater than hemoglobin. We note that the low molecular weight peak, the existence of which has been postulated, has been identified for the first time without the addition of a chelating agent. We speculate that the 17,000 mol wt peak is an α-chain pool.
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U2 - 10.1182/blood.v56.3.564.bloodjournal563564
DO - 10.1182/blood.v56.3.564.bloodjournal563564
M3 - Article
C2 - 7407419
AN - SCOPUS:0019213535
SN - 0006-4971
VL - 56
SP - 564
EP - 566
JO - Blood
JF - Blood
IS - 3
ER -