TY - JOUR
T1 - Loss ofp27Kip1 enhances the transplantation efficiency of hepatocytes transferred into diseased livers
AU - Karnezis, Anthony N.
AU - Dorokhov, Marina
AU - Grompe, Markus
AU - Zhu, Liang
PY - 2001
Y1 - 2001
N2 - p27kip1 is an important regulator of cyclin-dependent kinases. Studies with p27 knockout mice have revealed abnormalities in proliferation and differentiation of multiple cell types. Here we show that primary hepatocytes isolated from livers of adult p27 knockout mice exhibit higher levels of DNA synthesis activity in culture than do wild-type cells. Interestingly, we found that, compared with control hepatocytes, p27 knockout hepatocytes proliferate better after transplantation into diseased livers to reverse liver failure. These results reveal an aspect of p27 that could be used to benefit cell-based therapy.
AB - p27kip1 is an important regulator of cyclin-dependent kinases. Studies with p27 knockout mice have revealed abnormalities in proliferation and differentiation of multiple cell types. Here we show that primary hepatocytes isolated from livers of adult p27 knockout mice exhibit higher levels of DNA synthesis activity in culture than do wild-type cells. Interestingly, we found that, compared with control hepatocytes, p27 knockout hepatocytes proliferate better after transplantation into diseased livers to reverse liver failure. These results reveal an aspect of p27 that could be used to benefit cell-based therapy.
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U2 - 10.1172/JCI200111933
DO - 10.1172/JCI200111933
M3 - Article
C2 - 11489931
AN - SCOPUS:0034742547
SN - 0021-9738
VL - 108
SP - 383
EP - 390
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
IS - 3
ER -