Loss ofp27Kip1 enhances the transplantation efficiency of hepatocytes transferred into diseased livers

Anthony N. Karnezis, Marina Dorokhov, Markus Grompe, Liang Zhu

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

p27kip1 is an important regulator of cyclin-dependent kinases. Studies with p27 knockout mice have revealed abnormalities in proliferation and differentiation of multiple cell types. Here we show that primary hepatocytes isolated from livers of adult p27 knockout mice exhibit higher levels of DNA synthesis activity in culture than do wild-type cells. Interestingly, we found that, compared with control hepatocytes, p27 knockout hepatocytes proliferate better after transplantation into diseased livers to reverse liver failure. These results reveal an aspect of p27 that could be used to benefit cell-based therapy.

Original languageEnglish (US)
Pages (from-to)383-390
Number of pages8
JournalJournal of Clinical Investigation
Volume108
Issue number3
DOIs
StatePublished - 2001

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Liver Diseases
Hepatocytes
Transplantation
Knockout Mice
Cyclin-Dependent Kinases
Liver Failure
Cell- and Tissue-Based Therapy
Cell Differentiation
Liver
DNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Loss ofp27Kip1 enhances the transplantation efficiency of hepatocytes transferred into diseased livers. / Karnezis, Anthony N.; Dorokhov, Marina; Grompe, Markus; Zhu, Liang.

In: Journal of Clinical Investigation, Vol. 108, No. 3, 2001, p. 383-390.

Research output: Contribution to journalArticle

Karnezis, Anthony N. ; Dorokhov, Marina ; Grompe, Markus ; Zhu, Liang. / Loss ofp27Kip1 enhances the transplantation efficiency of hepatocytes transferred into diseased livers. In: Journal of Clinical Investigation. 2001 ; Vol. 108, No. 3. pp. 383-390.
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