Loss of polycystin-1 causes centrosome amplification and genomic instability

Lorenzo Battini, Salvador Macip, Elena Fedorova, Steven Dikman, Stefan Somlo, Cristina Montagna, G. Luca Gusella

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Autosomal dominant polycystic kidney disease (ADPKD) is the most common monogenetic disease predominantly caused by alteration or dysregulation of the PKD1 gene, which encodes polycystin-1 (PC1). The disease is characterized by the progressive expansion of bilateral fluid-filled renal cysts that ultimately lead to renal failure. Individual cysts, even within patients with germline mutations, are genetically heterogeneous, displaying diverse chromosomal abnormalities. To date, the molecular mechanisms responsible for this genetic heterogeneity remain unknown. Using a lentiviral-mediated siRNA expression model of Pkd1 hypomorphism, we show that loss of PC1 function is sufficient to produce centrosome amplification and multipolar spindle formation. These events lead to genomic instability characterized by gross polyploidism and mitotic catastrophe. Following these dramatic early changes, the cell population rapidly converges toward a stable ploidy in which centrosome amplification is significantly decreased, though cytological abnormalities such as micronucleation, chromatin bridges and aneuploidy remain common. In agreement with our in vitro findings, we provide the first in vivo evidence that significant centrosome amplification occurs in kidneys from conditional Pkd1 knockout mice at early and late time during the disease progression as well as in human ADPKD patients. These findings establish a novel function of PC1 in ADPKD pathogenesis and a genetic mechanism that may underlie the intrafamilial variability of ADPKD progression.

Original languageEnglish (US)
Pages (from-to)2819-2833
Number of pages15
JournalHuman Molecular Genetics
Volume17
Issue number18
DOIs
StatePublished - 2008

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Autosomal Dominant Polycystic Kidney
Centrosome
Genomic Instability
Disease Progression
Cysts
Kidney
Genetic Heterogeneity
Germ-Line Mutation
Ploidies
Aneuploidy
Knockout Mice
Chromosome Aberrations
Small Interfering RNA
Chromatin
Renal Insufficiency
polycystic kidney disease 1 protein
Population
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Battini, L., Macip, S., Fedorova, E., Dikman, S., Somlo, S., Montagna, C., & Gusella, G. L. (2008). Loss of polycystin-1 causes centrosome amplification and genomic instability. Human Molecular Genetics, 17(18), 2819-2833. https://doi.org/10.1093/hmg/ddn180

Loss of polycystin-1 causes centrosome amplification and genomic instability. / Battini, Lorenzo; Macip, Salvador; Fedorova, Elena; Dikman, Steven; Somlo, Stefan; Montagna, Cristina; Gusella, G. Luca.

In: Human Molecular Genetics, Vol. 17, No. 18, 2008, p. 2819-2833.

Research output: Contribution to journalArticle

Battini, L, Macip, S, Fedorova, E, Dikman, S, Somlo, S, Montagna, C & Gusella, GL 2008, 'Loss of polycystin-1 causes centrosome amplification and genomic instability', Human Molecular Genetics, vol. 17, no. 18, pp. 2819-2833. https://doi.org/10.1093/hmg/ddn180
Battini, Lorenzo ; Macip, Salvador ; Fedorova, Elena ; Dikman, Steven ; Somlo, Stefan ; Montagna, Cristina ; Gusella, G. Luca. / Loss of polycystin-1 causes centrosome amplification and genomic instability. In: Human Molecular Genetics. 2008 ; Vol. 17, No. 18. pp. 2819-2833.
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