Loss of imprinting of IGF2 and H19 in osteosarcoma is accompanied by reciprocal methylation changes of a CTCF-binding site

Gary A. Ulaner, Thanh H. Vu, Tao Li, Ji Fan Hu, Xiao Ming Yao, Youwen Yang, Richard Gorlick, Paul Meyers, John Healey, Marc Ladanyi, Andrew R. Hoffman

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

The adjacent insulin-like growth factor 2 (IGF2) and H19 genes are imprinted in most normal human tissues, but imprinting is often lost in tumors. The mechanisms involved in maintenance of imprinting (MOI) and loss of imprinting (LOI) are unresolved. We show here that osteosarcoma (OS) tumors with IGF2/H19 MOI exhibit allele-specific differential methylation of a CTCF-binding site upstream of H19. LOI of IGF2 or H19 in OS occurs in a mutually exclusive manner, and occurs with monoallelic expression of the other gene. Bisulfite sequencing reveals IGF2 LOI occurs with biallelic CpG methylation of the CTCF-binding site, while H19 LOI occurs with biallelic hypomethylation of this site. Our data demonstrate that IGF2 LOI and H19 LOI are accompanied by reciprocal methylation changes at a critical CTCF-binding site. We propose a model in which incomplete gain or loss of methylation at this CTCF-binding site during tumorigenesis explains the complex and often conflicting expression patterns of IGF2 and H19 in tumors.

Original languageEnglish (US)
Pages (from-to)535-549
Number of pages15
JournalHuman Molecular Genetics
Volume12
Issue number5
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

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Somatomedins
Osteosarcoma
Methylation
Binding Sites
Maintenance
Neoplasms
Carcinogenesis
Alleles
Gene Expression
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Loss of imprinting of IGF2 and H19 in osteosarcoma is accompanied by reciprocal methylation changes of a CTCF-binding site. / Ulaner, Gary A.; Vu, Thanh H.; Li, Tao; Hu, Ji Fan; Yao, Xiao Ming; Yang, Youwen; Gorlick, Richard; Meyers, Paul; Healey, John; Ladanyi, Marc; Hoffman, Andrew R.

In: Human Molecular Genetics, Vol. 12, No. 5, 01.03.2003, p. 535-549.

Research output: Contribution to journalArticle

Ulaner, GA, Vu, TH, Li, T, Hu, JF, Yao, XM, Yang, Y, Gorlick, R, Meyers, P, Healey, J, Ladanyi, M & Hoffman, AR 2003, 'Loss of imprinting of IGF2 and H19 in osteosarcoma is accompanied by reciprocal methylation changes of a CTCF-binding site', Human Molecular Genetics, vol. 12, no. 5, pp. 535-549. https://doi.org/10.1093/hmg/ddg034
Ulaner, Gary A. ; Vu, Thanh H. ; Li, Tao ; Hu, Ji Fan ; Yao, Xiao Ming ; Yang, Youwen ; Gorlick, Richard ; Meyers, Paul ; Healey, John ; Ladanyi, Marc ; Hoffman, Andrew R. / Loss of imprinting of IGF2 and H19 in osteosarcoma is accompanied by reciprocal methylation changes of a CTCF-binding site. In: Human Molecular Genetics. 2003 ; Vol. 12, No. 5. pp. 535-549.
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AU - Yang, Youwen

AU - Gorlick, Richard

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