Loss of hepatic chaperone-mediated autophagy accelerates proteostasis failure in aging

Jaime L. Schneider, Joan Villarroya, Antonio Diaz-Carretero, Bindi Patel, Aleksandra M. Urbanska, Mia M. Thi, Francesc Villarroya, Laura Santambrogio, Ana Maria Cuervo

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Chaperone-mediated autophagy (CMA), a cellular process that contributes to protein quality control through targeting of a subset of cytosolic proteins to lysosomes for degradation, undergoes a functional decline with age. We have used a mouse model with liver-specific defective CMA to identify changes in proteostasis attributable to reduced CMA activity in this organ with age. We have found that other proteolytic systems compensate for CMA loss in young mice which helps to preserve proteostasis. However, these compensatory responses are not sufficient for protection against proteotoxicity induced by stress (oxidative stress, lipid challenges) or associated with aging. Livers from old mice with CMA blockage exhibit altered protein homeostasis, enhanced susceptibility to oxidative stress and hepatic dysfunction manifested by a diminished ability to metabolize drugs, and a worsening of the metabolic dysregulation identified in young mice. Our study reveals that while the regulatory function of CMA cannot be compensated for in young organisms, its contribution to protein homeostasis can be handled by other proteolytic systems. However, the decline in the compensatory ability identified with age explains the more severe consequences of CMA impairment in older organisms and the contribution of CMA malfunction to the gradual decline in proteostasis and stress resistance observed during aging.

Original languageEnglish (US)
Pages (from-to)249-264
Number of pages16
JournalAging Cell
Volume14
Issue number2
DOIs
StatePublished - Apr 1 2015

Fingerprint

Autophagy
Liver
Proteins
Oxidative Stress
Homeostasis
Lysosomes
Quality Control
Lipids
Pharmaceutical Preparations

Keywords

  • Autophagy
  • Lysosomal protein degradation
  • Macroautophagy
  • Oxidative stress
  • Protein aggregation
  • Proteotoxicity
  • Ubiquitin-proteasome system

ASJC Scopus subject areas

  • Cell Biology
  • Aging

Cite this

Schneider, J. L., Villarroya, J., Diaz-Carretero, A., Patel, B., Urbanska, A. M., Thi, M. M., ... Cuervo, A. M. (2015). Loss of hepatic chaperone-mediated autophagy accelerates proteostasis failure in aging. Aging Cell, 14(2), 249-264. https://doi.org/10.1111/acel.12310

Loss of hepatic chaperone-mediated autophagy accelerates proteostasis failure in aging. / Schneider, Jaime L.; Villarroya, Joan; Diaz-Carretero, Antonio; Patel, Bindi; Urbanska, Aleksandra M.; Thi, Mia M.; Villarroya, Francesc; Santambrogio, Laura; Cuervo, Ana Maria.

In: Aging Cell, Vol. 14, No. 2, 01.04.2015, p. 249-264.

Research output: Contribution to journalArticle

Schneider, JL, Villarroya, J, Diaz-Carretero, A, Patel, B, Urbanska, AM, Thi, MM, Villarroya, F, Santambrogio, L & Cuervo, AM 2015, 'Loss of hepatic chaperone-mediated autophagy accelerates proteostasis failure in aging', Aging Cell, vol. 14, no. 2, pp. 249-264. https://doi.org/10.1111/acel.12310
Schneider JL, Villarroya J, Diaz-Carretero A, Patel B, Urbanska AM, Thi MM et al. Loss of hepatic chaperone-mediated autophagy accelerates proteostasis failure in aging. Aging Cell. 2015 Apr 1;14(2):249-264. https://doi.org/10.1111/acel.12310
Schneider, Jaime L. ; Villarroya, Joan ; Diaz-Carretero, Antonio ; Patel, Bindi ; Urbanska, Aleksandra M. ; Thi, Mia M. ; Villarroya, Francesc ; Santambrogio, Laura ; Cuervo, Ana Maria. / Loss of hepatic chaperone-mediated autophagy accelerates proteostasis failure in aging. In: Aging Cell. 2015 ; Vol. 14, No. 2. pp. 249-264.
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