Loss of Antigenic Epitopes as the Result of Env Gene Recombination in Retrovirus-Induced Leukemia in Immunocompetent Mice

Kathleen M. Tumas, Judith M. Poszgay, Nili Avidan, Stephen J. Ksiazek, Beth Overmoyer, Kenneth J. Blank, Michael B. Prystowsky

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The murine leukemia virus, E-55+ virus, induces a thymic lymphoma/leukemia in 100% of BALB.K mice infected as adults after a latent period of 4 months or more (Pozsgay et al., Virology 173, 330-334, 1989). Two molecular clones of virus designated E-55+ and E-55- based on their ability to encode the E-55 epitope detected by the monoclonal antibody 55 (mAb 55) were isolated from a leukemic BALB.K mouse inoculated with a biologically cloned E-55+ virus (Chesebro et al., Virology 112, 131-144, 1981). Env gene sequence analysis of E-55+ and E-55- clones showed that the E-55- virus was generated from the E-55+ virus as the result of a recombination between E-55+ virus and the endogenous ecotropic virus, emv-1, carried in the genome of the BALB.K mouse strain. The recombinant E-55- virus is replication competent. This recombination event and the consequential expression of E-55- virus consistently occur in immunocompetent BALB.K mice inoculated with the E-55+ virus and appear to play a role in the loss of epitopes recognized by virus neutralizing antibodies. The loss of these epitopes apparently allows the virus to evade the host immune response.

Original languageEnglish (US)
Pages (from-to)587-595
Number of pages9
JournalVirology
Volume192
Issue number2
DOIs
StatePublished - Feb 1993
Externally publishedYes

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env Genes
Retroviridae
Genetic Recombination
Epitopes
Leukemia
Viruses
Virology
Clone Cells
Murine Leukemia Viruses
Virus Replication
Neutralizing Antibodies
Sequence Analysis
Lymphoma
Monoclonal Antibodies

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Loss of Antigenic Epitopes as the Result of Env Gene Recombination in Retrovirus-Induced Leukemia in Immunocompetent Mice. / Tumas, Kathleen M.; Poszgay, Judith M.; Avidan, Nili; Ksiazek, Stephen J.; Overmoyer, Beth; Blank, Kenneth J.; Prystowsky, Michael B.

In: Virology, Vol. 192, No. 2, 02.1993, p. 587-595.

Research output: Contribution to journalArticle

Tumas, Kathleen M. ; Poszgay, Judith M. ; Avidan, Nili ; Ksiazek, Stephen J. ; Overmoyer, Beth ; Blank, Kenneth J. ; Prystowsky, Michael B. / Loss of Antigenic Epitopes as the Result of Env Gene Recombination in Retrovirus-Induced Leukemia in Immunocompetent Mice. In: Virology. 1993 ; Vol. 192, No. 2. pp. 587-595.
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