Loss of allograft inflammatory factor-1 ameliorates experimental autoimmune encephalomyelitis by limiting encephalitogenic CD4 T-cell expansion

Prameladevi Chinnasamy, Sarah E. Lutz, Dario F. Riascos Bernal, Venkatesh Jeganathan, Isabel Casimiro, Celia F. Brosnan, Nicholas E.S. Sibinga

Research output: Contribution to journalArticle

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Abstract

Experimental autoimmune encephalomyelitis (EAE), an animal model of human multiple sclerosis (MS), is mediated by myelin- specific auto reactive T cells that cause inflammation and demyelination in the central nervous system (CNS), with significant contributions from activated microglia and macrophages. The molecular bases for expansion and activation of these cells, plus traf- ficking to the CNS for peripheral cells, are not fully understood. Allograft inflammatory factor-1 (Aif-1) (also known as ionized Ca<sup>2+</sup> binding adapter-1 [Iba-1]) is induced in leukocytes in MS and EAE; here we provide the first assessment of Aif-1 function in this setting. After myelin oligodendrocyte glycoprotein peptide (MOG<inf>35-55</inf>) immunization, Aif-1-deficient mice were less likely than controls to develop EAE and had less CNS leukocyte infiltration and demyelination; their spinal cords contained fewer CD4 T cells and microglia and more CD8 T cells. These mice also showed significantly less splenic CD4 T-cell expansion and activation, plus de- creased proinflammatory cytokine expression. These findings identify Aif-1 as a potent molecule that promotes expansion and activation of CD4 T cells, plus elaboration of a proinflammatory cytokine milieu, in MOG35-55-induced EAE and as a potential therapeutic target in MS.

Original languageEnglish (US)
Pages (from-to)233-241
Number of pages9
JournalMolecular Medicine
Volume21
DOIs
StatePublished - 2015

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Autoimmune Experimental Encephalomyelitis
Allografts
T-Lymphocytes
Multiple Sclerosis
Central Nervous System
Microglia
Demyelinating Diseases
Leukocytes
Myelin-Oligodendrocyte Glycoprotein
Cytokines
Myelin Sheath
Immunization
Spinal Cord
Animal Models
Macrophages
Inflammation
Peptides

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Genetics(clinical)

Cite this

Loss of allograft inflammatory factor-1 ameliorates experimental autoimmune encephalomyelitis by limiting encephalitogenic CD4 T-cell expansion. / Chinnasamy, Prameladevi; Lutz, Sarah E.; Riascos Bernal, Dario F.; Jeganathan, Venkatesh; Casimiro, Isabel; Brosnan, Celia F.; Sibinga, Nicholas E.S.

In: Molecular Medicine, Vol. 21, 2015, p. 233-241.

Research output: Contribution to journalArticle

Chinnasamy, Prameladevi ; Lutz, Sarah E. ; Riascos Bernal, Dario F. ; Jeganathan, Venkatesh ; Casimiro, Isabel ; Brosnan, Celia F. ; Sibinga, Nicholas E.S. / Loss of allograft inflammatory factor-1 ameliorates experimental autoimmune encephalomyelitis by limiting encephalitogenic CD4 T-cell expansion. In: Molecular Medicine. 2015 ; Vol. 21. pp. 233-241.
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