Longitudinal multiparametric MRI study of hydrogen-enriched water with minocycline combination therapy in experimental ischemic stroke in rats

Zhao Jiang; Tharun T. Alamuri; Eric R. Muir; Dennis W. Choi; Tim Q. Duong

doi:10.1016/j.brainres.2020.147122

Longitudinal multiparametric MRI study of hydrogen-enriched water with minocycline combination therapy in experimental ischemic stroke in rats

Zhao Jiang, Tharun T. Alamuri, Eric R. Muir, Dennis W. Choi, Tim Q. Duong

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Free radicals are downstream mediators of several cytotoxic cascades contributing to ischemic brain injury. Molecular hydrogen (H₂) is an antioxidant potentially useful in the treatment of stroke. Hydrogen is easy to deliver, biologically non-toxic and diffuses freely through all biological structures including the blood-brain barrier and cellular membranes. This study evaluated the efficacy of hydrogen treatments in a rat stroke model compared to vehicle-treated controls using multiparametric MRI and neurological tests. Additionally, comparison of H₂ treatment alone was made with H₂ combined with minocycline (H2M) treatment (12 rats per group). The primary findings were: i) H₂ therapy reduced infarct volume in both H₂ and H2M groups compared to controls at 1 and 7 days after stroke, and ii) both H₂ and H2M improved neurologic functional recovery on day 7. The secondary outcomes were: iii) H2M treatment attenuated post-stroke hyperperfusion in the hyperacute phase, and iv) H2M markedly minimized white matter injury. In conclusion, this is the first study to use MRI to longitudinally study H₂ and H2M treatment on ischemic stroke and the first study to compare H₂ treatment combined with another potential stroke therapeutic (H2M).

Original language	English (US)
Article number	147122
Journal	Brain research
Volume	1748
DOIs	https://doi.org/10.1016/j.brainres.2020.147122
State	Published - Dec 1 2020
Externally published	Yes

Keywords

Antioxidant
Cerebral blow flow
Diffusion tensor imaging
Hydrogen
Neuroprotection
Stroke

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/j.brainres.2020.147122

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title = "Longitudinal multiparametric MRI study of hydrogen-enriched water with minocycline combination therapy in experimental ischemic stroke in rats",

abstract = "Free radicals are downstream mediators of several cytotoxic cascades contributing to ischemic brain injury. Molecular hydrogen (H2) is an antioxidant potentially useful in the treatment of stroke. Hydrogen is easy to deliver, biologically non-toxic and diffuses freely through all biological structures including the blood-brain barrier and cellular membranes. This study evaluated the efficacy of hydrogen treatments in a rat stroke model compared to vehicle-treated controls using multiparametric MRI and neurological tests. Additionally, comparison of H2 treatment alone was made with H2 combined with minocycline (H2M) treatment (12 rats per group). The primary findings were: i) H2 therapy reduced infarct volume in both H2 and H2M groups compared to controls at 1 and 7 days after stroke, and ii) both H2 and H2M improved neurologic functional recovery on day 7. The secondary outcomes were: iii) H2M treatment attenuated post-stroke hyperperfusion in the hyperacute phase, and iv) H2M markedly minimized white matter injury. In conclusion, this is the first study to use MRI to longitudinally study H2 and H2M treatment on ischemic stroke and the first study to compare H2 treatment combined with another potential stroke therapeutic (H2M).",

keywords = "Antioxidant, Cerebral blow flow, Diffusion tensor imaging, Hydrogen, Neuroprotection, Stroke",

author = "Zhao Jiang and Alamuri, {Tharun T.} and Muir, {Eric R.} and Choi, {Dennis W.} and Duong, {Tim Q.}",

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doi = "10.1016/j.brainres.2020.147122",

language = "English (US)",

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AU - Jiang, Zhao

AU - Alamuri, Tharun T.

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AU - Choi, Dennis W.

AU - Duong, Tim Q.

PY - 2020/12/1

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AB - Free radicals are downstream mediators of several cytotoxic cascades contributing to ischemic brain injury. Molecular hydrogen (H2) is an antioxidant potentially useful in the treatment of stroke. Hydrogen is easy to deliver, biologically non-toxic and diffuses freely through all biological structures including the blood-brain barrier and cellular membranes. This study evaluated the efficacy of hydrogen treatments in a rat stroke model compared to vehicle-treated controls using multiparametric MRI and neurological tests. Additionally, comparison of H2 treatment alone was made with H2 combined with minocycline (H2M) treatment (12 rats per group). The primary findings were: i) H2 therapy reduced infarct volume in both H2 and H2M groups compared to controls at 1 and 7 days after stroke, and ii) both H2 and H2M improved neurologic functional recovery on day 7. The secondary outcomes were: iii) H2M treatment attenuated post-stroke hyperperfusion in the hyperacute phase, and iv) H2M markedly minimized white matter injury. In conclusion, this is the first study to use MRI to longitudinally study H2 and H2M treatment on ischemic stroke and the first study to compare H2 treatment combined with another potential stroke therapeutic (H2M).

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KW - Neuroprotection

KW - Stroke

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Longitudinal multiparametric MRI study of hydrogen-enriched water with minocycline combination therapy in experimental ischemic stroke in rats

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Keywords

ASJC Scopus subject areas

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