Long term storage of virus templated fluorescent materials for sensing applications

Raviraja N. Seetharam, Amy Szuchmacher Blum, Carissa M. Soto, Jessica L. Whitley, Kim E. Sapsford, Anju Chatterji, Tianwei Lin, John E. Johnson, Charles Guerra, Peter Satir, Banahalli R. Ratna

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Wild type, mutant, and chemically modified Cowpea mosaic viruses (CPMV) were studied for long term preservation in the presence and absence of cryoprotectants. Viral complexes were reconstituted and tested via fluorescence spectroscopy and a UV/vis-based RNase assay for structural integrity. When viruses lyophilized in the absence of cryoprotectant were rehydrated and RNase treated, UV absorption increased, indicating that the capsids were damaged. The addition of trehalose during lyophilization protected capsid integrity for at least 7 weeks. Measurements of the fluorescence peak maximum of CPMV lyophilized with trehalose and reconstituted also indicate that the virus remained intact. Microarray binding assays indicated that CPMV particles chemically modified for use as a fluorescent tracer were intact and retained binding specificity after lyophilization in the presence of trehalose. Thus, we demonstrate that functionalized CPMV nanostructures can be stored for the long term, enabling their use in practical sensing applications.

Original languageEnglish (US)
Article number105504
JournalNanotechnology
Volume19
Issue number10
DOIs
StatePublished - Mar 12 2008

Fingerprint

Viruses
Trehalose
Ribonucleases
Assays
Fluorescence spectroscopy
Structural integrity
Microarrays
Nanostructures
Fluorescence

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Seetharam, R. N., Szuchmacher Blum, A., Soto, C. M., Whitley, J. L., Sapsford, K. E., Chatterji, A., ... Ratna, B. R. (2008). Long term storage of virus templated fluorescent materials for sensing applications. Nanotechnology, 19(10), [105504]. https://doi.org/10.1088/0957-4484/19/10/105504

Long term storage of virus templated fluorescent materials for sensing applications. / Seetharam, Raviraja N.; Szuchmacher Blum, Amy; Soto, Carissa M.; Whitley, Jessica L.; Sapsford, Kim E.; Chatterji, Anju; Lin, Tianwei; Johnson, John E.; Guerra, Charles; Satir, Peter; Ratna, Banahalli R.

In: Nanotechnology, Vol. 19, No. 10, 105504, 12.03.2008.

Research output: Contribution to journalArticle

Seetharam, RN, Szuchmacher Blum, A, Soto, CM, Whitley, JL, Sapsford, KE, Chatterji, A, Lin, T, Johnson, JE, Guerra, C, Satir, P & Ratna, BR 2008, 'Long term storage of virus templated fluorescent materials for sensing applications', Nanotechnology, vol. 19, no. 10, 105504. https://doi.org/10.1088/0957-4484/19/10/105504
Seetharam RN, Szuchmacher Blum A, Soto CM, Whitley JL, Sapsford KE, Chatterji A et al. Long term storage of virus templated fluorescent materials for sensing applications. Nanotechnology. 2008 Mar 12;19(10). 105504. https://doi.org/10.1088/0957-4484/19/10/105504
Seetharam, Raviraja N. ; Szuchmacher Blum, Amy ; Soto, Carissa M. ; Whitley, Jessica L. ; Sapsford, Kim E. ; Chatterji, Anju ; Lin, Tianwei ; Johnson, John E. ; Guerra, Charles ; Satir, Peter ; Ratna, Banahalli R. / Long term storage of virus templated fluorescent materials for sensing applications. In: Nanotechnology. 2008 ; Vol. 19, No. 10.
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