Long-term engraftment and function of transplanted pancreatic islets in vascularized segments of small intestine

Zurab Kakabadze, Sanjeev Gupta, Daniel Brandhorst, Olle Korsgren, Ekaterine Berishvili

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This study evaluated the potential of vascularized small intestinal segments for pancreatic islet transplantation. Islets isolated from Lewis rats were transplanted into diabetic syngeneic recipients. Segments of small intestine were prepared by denudation of the mucosal layer prior to implantation of pancreatic islets into the segments. Animal groups were established to determine engraftment, survival and function of islets transplanted into either intestinal segments or portal vein over up to 60 days. We found transplantation of functionally intact pancreatic islets into small intestinal segments was well tolerated. Transplanted islets were rapidly engrafted in intestinal segments as demonstrated vascularization and expression of insulin and glucagon throughout the 60-day duration of the studies. Transplantation of islets restored euglycemia in diabetic rats, which was similar to animals receiving islets intraportally. Moreover, animals treated with islet transplants showed normal responses to glucose challenges. Removal of graft-bearing intestinal segments led to recurrence of hyperglycemia indicating that transplanted islets were responsible for improved outcomes. Therefore, we concluded that vascularized intestinal segments supported reorganization, survival and function of transplanted islets with therapeutic efficacy in streptozotocin-treated diabetic rats. The approach described here will be appropriate for studying islet biogenesis, reorganization and function, including for cell therapy applications.

Original languageEnglish (US)
Pages (from-to)175-183
Number of pages9
JournalTransplant International
Volume24
Issue number2
DOIs
StatePublished - Feb 2011

Fingerprint

Islets of Langerhans
Small Intestine
Islets of Langerhans Transplantation
Transplants
Streptozocin
Cell- and Tissue-Based Therapy
Portal Vein
Glucagon
Hyperglycemia
Transplantation
Insulin
Glucose
Recurrence
Therapeutics

Keywords

  • islets
  • pancreas
  • portal vein
  • small intestine
  • transplantation

ASJC Scopus subject areas

  • Transplantation

Cite this

Long-term engraftment and function of transplanted pancreatic islets in vascularized segments of small intestine. / Kakabadze, Zurab; Gupta, Sanjeev; Brandhorst, Daniel; Korsgren, Olle; Berishvili, Ekaterine.

In: Transplant International, Vol. 24, No. 2, 02.2011, p. 175-183.

Research output: Contribution to journalArticle

Kakabadze, Zurab ; Gupta, Sanjeev ; Brandhorst, Daniel ; Korsgren, Olle ; Berishvili, Ekaterine. / Long-term engraftment and function of transplanted pancreatic islets in vascularized segments of small intestine. In: Transplant International. 2011 ; Vol. 24, No. 2. pp. 175-183.
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