Long-term depression of presynaptic release from the readily releasable vesicle pool induced by NMDA receptor-dependent retrograde nitric oxide

Patric K. Stanton, Jochen Winterer, Christopher P. Bailey, Andreas Kyrozis, Ivan Raginov, Gregor Laube, Rüdiger W. Veh, Ken C.Q. Nguyen, Wolfgang Müller

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Postsynaptic alterations are currently believed to be able to fully account for NMDA-receptor-dependent long-term depression (LTD) and long-term potentiation of synaptic strength, although there is also evidence supporting changes in presynaptic release. Using dual-photon laser scan microscopy of N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino)styryl) pyridinium dibromide (FM1-43) to directly visualize presynaptic vesicular release at Schaffer collateral-CA1 excitatory synapses in hippocampal slices, we demonstrate reduced vesicular release associated with LTD. Selective loading, byhypertonic shock, of the readily releasable vesicle pool (RRP) showed that LTD of release is a selective modification of release from the RRP. Presynaptic LTD of RRP release required activation of NMDA receptors, production and extracellular diffusion of the intercellular messenger NO, and activation of cGMP-dependent protein kinase.

Original languageEnglish (US)
Pages (from-to)5936-5944
Number of pages9
JournalJournal of Neuroscience
Volume23
Issue number13
StatePublished - Jul 1 2003

Fingerprint

N-Methyl-D-Aspartate Receptors
Nitric Oxide
Cyclic GMP-Dependent Protein Kinases
Long-Term Potentiation
Photons
Confocal Microscopy
Synapses
Shock
Hippocampus
FM1 43

Keywords

  • CA1
  • CGMP
  • Hippocampus
  • Long-term depression
  • Nitric oxide
  • PKG
  • Presynaptic
  • Readily releasable vesicle pool
  • Schaffer collateral
  • Transmitter release

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Stanton, P. K., Winterer, J., Bailey, C. P., Kyrozis, A., Raginov, I., Laube, G., ... Müller, W. (2003). Long-term depression of presynaptic release from the readily releasable vesicle pool induced by NMDA receptor-dependent retrograde nitric oxide. Journal of Neuroscience, 23(13), 5936-5944.

Long-term depression of presynaptic release from the readily releasable vesicle pool induced by NMDA receptor-dependent retrograde nitric oxide. / Stanton, Patric K.; Winterer, Jochen; Bailey, Christopher P.; Kyrozis, Andreas; Raginov, Ivan; Laube, Gregor; Veh, Rüdiger W.; Nguyen, Ken C.Q.; Müller, Wolfgang.

In: Journal of Neuroscience, Vol. 23, No. 13, 01.07.2003, p. 5936-5944.

Research output: Contribution to journalArticle

Stanton, PK, Winterer, J, Bailey, CP, Kyrozis, A, Raginov, I, Laube, G, Veh, RW, Nguyen, KCQ & Müller, W 2003, 'Long-term depression of presynaptic release from the readily releasable vesicle pool induced by NMDA receptor-dependent retrograde nitric oxide', Journal of Neuroscience, vol. 23, no. 13, pp. 5936-5944.
Stanton, Patric K. ; Winterer, Jochen ; Bailey, Christopher P. ; Kyrozis, Andreas ; Raginov, Ivan ; Laube, Gregor ; Veh, Rüdiger W. ; Nguyen, Ken C.Q. ; Müller, Wolfgang. / Long-term depression of presynaptic release from the readily releasable vesicle pool induced by NMDA receptor-dependent retrograde nitric oxide. In: Journal of Neuroscience. 2003 ; Vol. 23, No. 13. pp. 5936-5944.
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