Long non-coding RNA regulates hair follicle stem cell proliferation and differentiation through PI3K/AKT signal pathway

Bingjie Cai, Yunpeng Zheng, Shanshan Ma, Qu Xing, Xinxin Wang, Bo Yang, Guangwen Yin, Fangxia Guan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Long non-coding RNAs (lncRNAs) are defined as non-coding transcripts (>200 nucleotides) that serve important roles in the proliferation and differentiation of stem cells. Hair follicle stem cells (HFTs) have multidirectional differentiation potential and are able to differentiate into skin, hair follicles and sebaceous glands, serving a role in skin wound healing. The aim of the present study was to analyze the regulatory role of lncRNA AK015322 (IncRNA5322) in HFTs and the potential mechanism of IncRNA5322-mediated differentiation of HFTs. The results demonstrated that lncRNA5322 transfection promoted proliferation and differentiation in HFTs. It was identified that lncRNA5322 transfection upregulated the expression and phosphorylation of phosphoinositide 3-kinase (PI3K) and protein kinase B (AKT) in HFTs. It was also observed that lncRNA5322 transfection upregulated microRNA (miR)-21 and miR-21 agonist (agomir-21) eliminated lncRNA5322-induced expression and phosphorylation of PI3K and AKT. The present study also demonstrated that agomir-21 blocked IncRNA5322-induced expression and phosphorylation of PI3K and AKT in HFTs. The results indicated that agomir-21 transfection also suppressed the IncRNA5322-induced proliferation and differentiation of HFTs. In conclusion, the results of the present study suggest that lncRNA5322 is able to promote the proliferation and differentiation of HFTs by targeting the miR-21-mediated PI3K-AKT signaling pathway in HFTs.

Original languageEnglish (US)
Pages (from-to)5477-5483
Number of pages7
JournalMolecular Medicine Reports
Volume17
Issue number4
DOIs
StatePublished - Apr 1 2018
Externally publishedYes

Fingerprint

Long Noncoding RNA
1-Phosphatidylinositol 4-Kinase
Hair Follicle
Cell proliferation
Phosphatidylinositols
Stem cells
Phosphorylation
Transfection
Cell Differentiation
Signal Transduction
Phosphotransferases
Stem Cells
MicroRNAs
Cell Proliferation
Skin
Sebaceous Glands
Proto-Oncogene Proteins c-akt
Wound Healing
Nucleotides

Keywords

  • Differentiation
  • Hair follicle stem cells
  • Long non-coding RNA5322
  • MicroRNA-21
  • Phosphoinositide 3-kinase
  • Proliferation
  • Protein kinase B

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Oncology
  • Cancer Research

Cite this

Long non-coding RNA regulates hair follicle stem cell proliferation and differentiation through PI3K/AKT signal pathway. / Cai, Bingjie; Zheng, Yunpeng; Ma, Shanshan; Xing, Qu; Wang, Xinxin; Yang, Bo; Yin, Guangwen; Guan, Fangxia.

In: Molecular Medicine Reports, Vol. 17, No. 4, 01.04.2018, p. 5477-5483.

Research output: Contribution to journalArticle

Cai, Bingjie ; Zheng, Yunpeng ; Ma, Shanshan ; Xing, Qu ; Wang, Xinxin ; Yang, Bo ; Yin, Guangwen ; Guan, Fangxia. / Long non-coding RNA regulates hair follicle stem cell proliferation and differentiation through PI3K/AKT signal pathway. In: Molecular Medicine Reports. 2018 ; Vol. 17, No. 4. pp. 5477-5483.
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AU - Wang, Xinxin

AU - Yang, Bo

AU - Yin, Guangwen

AU - Guan, Fangxia

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AB - Long non-coding RNAs (lncRNAs) are defined as non-coding transcripts (>200 nucleotides) that serve important roles in the proliferation and differentiation of stem cells. Hair follicle stem cells (HFTs) have multidirectional differentiation potential and are able to differentiate into skin, hair follicles and sebaceous glands, serving a role in skin wound healing. The aim of the present study was to analyze the regulatory role of lncRNA AK015322 (IncRNA5322) in HFTs and the potential mechanism of IncRNA5322-mediated differentiation of HFTs. The results demonstrated that lncRNA5322 transfection promoted proliferation and differentiation in HFTs. It was identified that lncRNA5322 transfection upregulated the expression and phosphorylation of phosphoinositide 3-kinase (PI3K) and protein kinase B (AKT) in HFTs. It was also observed that lncRNA5322 transfection upregulated microRNA (miR)-21 and miR-21 agonist (agomir-21) eliminated lncRNA5322-induced expression and phosphorylation of PI3K and AKT. The present study also demonstrated that agomir-21 blocked IncRNA5322-induced expression and phosphorylation of PI3K and AKT in HFTs. The results indicated that agomir-21 transfection also suppressed the IncRNA5322-induced proliferation and differentiation of HFTs. In conclusion, the results of the present study suggest that lncRNA5322 is able to promote the proliferation and differentiation of HFTs by targeting the miR-21-mediated PI3K-AKT signaling pathway in HFTs.

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