Loci for CETP, LPL, LIPC, and APOC3 affect plasma lipoprotein size and subpopulation distribution in Hispanic and non-Hispanic white subjects

The Columbia University BioMarkers Study

S. E. Humphries, L. Berglund, Carmen R. Isasi, J. D. Otvos, D. Kaluski, R. J. Deckelbaum, S. Shea, P. J. Talmud

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background and Aim: The effect of genetic variation on plasma lipoproteins and their subfraction distribution was examined. Methods and Results: Forty Hispanic men and 223 women and 42 non-Hispanic white men and 53 women participated in the study. Genotypes for cholesteryl ester transfer protein (CETP TaqIB), hepatic lipase (LIPC-480C>T), lipoprotein lipase (LPL S447X), and apolipoprotein CIII (APOC3 -455T>C) were determined by polymerase chain reaction. Lipoprotein particle size distribution was determined by nuclear magnetic resonance. For all but APOC3, genotype effects were homogeneous in the ethnic/racial groups and men and women. Effects were seen primarily in the women. Compared to women carriers of the common CETP B1 allele, B2B2 women had significantly higher plasma levels of high-density lipoprotein cholesterol (HDL-C) (16.4.0%, p=0.001), reflected in the level of larger HDL particles (21.9%, p=0.001), and larger mean particle size of HDL (2.3%, p=0.01) and low-density lipoproteins (LDL) (1.3%, p=0.02). Compared to LPL 447S homozygous women carriers of the LPL 447X allele had significantly lower levels of very-low-density lipoprotein-triglyceride (VLDL-TG) (21.0%, p=0.02). For APOC3, there was significant gender:genotype interaction with the genotype differences seen only in the men. Compared to men homozygous for the -455T allele, carriers of -455C had higher levels of VLDL-TG (71.4%, p=0.0001), reflected in a larger mean VLDL particle size (13.7%, p=0.009). LIPC genotype was not associated with significant effects on any of these traits. Conclusion: These data confirm the role of genetic variants of CETP, LPL and APOC3 in determining the relationship between VLDL, LDL and HDL particles.

Original languageEnglish (US)
Pages (from-to)163-172
Number of pages10
JournalNutrition, Metabolism and Cardiovascular Diseases
Volume12
Issue number4
StatePublished - Aug 2002
Externally publishedYes

Fingerprint

Hispanic Americans
lipoproteins
Lipoproteins
biomarkers
Biomarkers
loci
Genotype
Particle Size
genotype
very low density lipoprotein
Alleles
low density lipoprotein
alleles
LDL Lipoproteins
particle size
triacylglycerols
cholesteryl ester transfer protein
Apolipoprotein C-III
Cholesterol Ester Transfer Proteins
apolipoproteins

Keywords

  • Genetic association
  • Lipoprotein size determination
  • NMR

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Cardiology and Cardiovascular Medicine
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Loci for CETP, LPL, LIPC, and APOC3 affect plasma lipoprotein size and subpopulation distribution in Hispanic and non-Hispanic white subjects : The Columbia University BioMarkers Study. / Humphries, S. E.; Berglund, L.; Isasi, Carmen R.; Otvos, J. D.; Kaluski, D.; Deckelbaum, R. J.; Shea, S.; Talmud, P. J.

In: Nutrition, Metabolism and Cardiovascular Diseases, Vol. 12, No. 4, 08.2002, p. 163-172.

Research output: Contribution to journalArticle

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title = "Loci for CETP, LPL, LIPC, and APOC3 affect plasma lipoprotein size and subpopulation distribution in Hispanic and non-Hispanic white subjects: The Columbia University BioMarkers Study",
abstract = "Background and Aim: The effect of genetic variation on plasma lipoproteins and their subfraction distribution was examined. Methods and Results: Forty Hispanic men and 223 women and 42 non-Hispanic white men and 53 women participated in the study. Genotypes for cholesteryl ester transfer protein (CETP TaqIB), hepatic lipase (LIPC-480C>T), lipoprotein lipase (LPL S447X), and apolipoprotein CIII (APOC3 -455T>C) were determined by polymerase chain reaction. Lipoprotein particle size distribution was determined by nuclear magnetic resonance. For all but APOC3, genotype effects were homogeneous in the ethnic/racial groups and men and women. Effects were seen primarily in the women. Compared to women carriers of the common CETP B1 allele, B2B2 women had significantly higher plasma levels of high-density lipoprotein cholesterol (HDL-C) (16.4.0{\%}, p=0.001), reflected in the level of larger HDL particles (21.9{\%}, p=0.001), and larger mean particle size of HDL (2.3{\%}, p=0.01) and low-density lipoproteins (LDL) (1.3{\%}, p=0.02). Compared to LPL 447S homozygous women carriers of the LPL 447X allele had significantly lower levels of very-low-density lipoprotein-triglyceride (VLDL-TG) (21.0{\%}, p=0.02). For APOC3, there was significant gender:genotype interaction with the genotype differences seen only in the men. Compared to men homozygous for the -455T allele, carriers of -455C had higher levels of VLDL-TG (71.4{\%}, p=0.0001), reflected in a larger mean VLDL particle size (13.7{\%}, p=0.009). LIPC genotype was not associated with significant effects on any of these traits. Conclusion: These data confirm the role of genetic variants of CETP, LPL and APOC3 in determining the relationship between VLDL, LDL and HDL particles.",
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TY - JOUR

T1 - Loci for CETP, LPL, LIPC, and APOC3 affect plasma lipoprotein size and subpopulation distribution in Hispanic and non-Hispanic white subjects

T2 - The Columbia University BioMarkers Study

AU - Humphries, S. E.

AU - Berglund, L.

AU - Isasi, Carmen R.

AU - Otvos, J. D.

AU - Kaluski, D.

AU - Deckelbaum, R. J.

AU - Shea, S.

AU - Talmud, P. J.

PY - 2002/8

Y1 - 2002/8

N2 - Background and Aim: The effect of genetic variation on plasma lipoproteins and their subfraction distribution was examined. Methods and Results: Forty Hispanic men and 223 women and 42 non-Hispanic white men and 53 women participated in the study. Genotypes for cholesteryl ester transfer protein (CETP TaqIB), hepatic lipase (LIPC-480C>T), lipoprotein lipase (LPL S447X), and apolipoprotein CIII (APOC3 -455T>C) were determined by polymerase chain reaction. Lipoprotein particle size distribution was determined by nuclear magnetic resonance. For all but APOC3, genotype effects were homogeneous in the ethnic/racial groups and men and women. Effects were seen primarily in the women. Compared to women carriers of the common CETP B1 allele, B2B2 women had significantly higher plasma levels of high-density lipoprotein cholesterol (HDL-C) (16.4.0%, p=0.001), reflected in the level of larger HDL particles (21.9%, p=0.001), and larger mean particle size of HDL (2.3%, p=0.01) and low-density lipoproteins (LDL) (1.3%, p=0.02). Compared to LPL 447S homozygous women carriers of the LPL 447X allele had significantly lower levels of very-low-density lipoprotein-triglyceride (VLDL-TG) (21.0%, p=0.02). For APOC3, there was significant gender:genotype interaction with the genotype differences seen only in the men. Compared to men homozygous for the -455T allele, carriers of -455C had higher levels of VLDL-TG (71.4%, p=0.0001), reflected in a larger mean VLDL particle size (13.7%, p=0.009). LIPC genotype was not associated with significant effects on any of these traits. Conclusion: These data confirm the role of genetic variants of CETP, LPL and APOC3 in determining the relationship between VLDL, LDL and HDL particles.

AB - Background and Aim: The effect of genetic variation on plasma lipoproteins and their subfraction distribution was examined. Methods and Results: Forty Hispanic men and 223 women and 42 non-Hispanic white men and 53 women participated in the study. Genotypes for cholesteryl ester transfer protein (CETP TaqIB), hepatic lipase (LIPC-480C>T), lipoprotein lipase (LPL S447X), and apolipoprotein CIII (APOC3 -455T>C) were determined by polymerase chain reaction. Lipoprotein particle size distribution was determined by nuclear magnetic resonance. For all but APOC3, genotype effects were homogeneous in the ethnic/racial groups and men and women. Effects were seen primarily in the women. Compared to women carriers of the common CETP B1 allele, B2B2 women had significantly higher plasma levels of high-density lipoprotein cholesterol (HDL-C) (16.4.0%, p=0.001), reflected in the level of larger HDL particles (21.9%, p=0.001), and larger mean particle size of HDL (2.3%, p=0.01) and low-density lipoproteins (LDL) (1.3%, p=0.02). Compared to LPL 447S homozygous women carriers of the LPL 447X allele had significantly lower levels of very-low-density lipoprotein-triglyceride (VLDL-TG) (21.0%, p=0.02). For APOC3, there was significant gender:genotype interaction with the genotype differences seen only in the men. Compared to men homozygous for the -455T allele, carriers of -455C had higher levels of VLDL-TG (71.4%, p=0.0001), reflected in a larger mean VLDL particle size (13.7%, p=0.009). LIPC genotype was not associated with significant effects on any of these traits. Conclusion: These data confirm the role of genetic variants of CETP, LPL and APOC3 in determining the relationship between VLDL, LDL and HDL particles.

KW - Genetic association

KW - Lipoprotein size determination

KW - NMR

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JO - Nutrition, Metabolism and Cardiovascular Diseases

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