Localizing DNA and RNA within nuclei and chromosomes by fluorescence in situ hybridization

John A. McNeil, Carol Villnave Johnson, Kenneth C. Carter, Robert H. Singer, Jeanne Bentley Lawrence

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

The enormous potential of in situ hybridization derives from the unique ability of this approach to directly couple cytological and molecular information. In recent years, there has been a surge of success in powerful new applications, resulting from methodologic advances that bring the practical capabilities of this technology closer to its theoretical potential. A major advance has been improvements that enable, with a high degree of reproducibility and efficiency, precise visualization of single sequences within individual metaphase and interphase cells. This has implications for gene mapping, the analysis of nuclear organization, clinical cytogenetics, virology, and studies of gene expression. This article discusses the current state of the art of fluorescence in situ hybridization, with emphasis on applications to human genetics, but including brief discussions on studies of nuclear DNA and RNA organization, and on applications to clinical genetics and virology. Although a review of all of the literature in this field is not possible here, many of the major contributions are summarized along with recent work from our laboratory.

Original languageEnglish (US)
Pages (from-to)41-58
Number of pages18
JournalGenetic Analysis: Biomolecular Engineering
Volume8
Issue number2
DOIs
StatePublished - Apr 1991
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

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